The Cause Of The Development Of Embryonic Development Is Based Onthe Combination Of Immuno-antibody And Partial Environmental Factoes

Objective:To discusses the types of villi chromosome abnormality in patients with dysplasia,and analyzes the etiology of the embryos in combination with the risk factors of some environmental risk factors.Methods:1 Reading a large number of documents,references in the literature after logistic regression analysis of the effects on embryonic diapause have some common environmental risk factors for the development of survey questionnaires;For mentors and experts,the comments and suggestions,through the expert,initially modified questionnaire;in December 2015 in the hospital's obstetrics and Gynecology outpatient clinic on embryonic development in patients with preliminary investigations,according to the survey results,deletes from or revises part of the problem,adjust the structure,according to the questionnaire further exposed in the process improved,formal questionnaire.The standard training of investigators,which monitor patients carefully fill in the questionnaire;On embryonic development of selected patients with basic statistics,including maternal age,gravidity,the stream number,number of embryonic diapause,last menstrual period,occupation,physical exercise a week number,daily use of cell phones and computers,before pregnancy and during pregnancy renovation history,medication history,mental stress,stay up late,work pressure and so on.2 Collected embryos from peripheral blood of patients with 3ml,placed after the separation of serum-20 refrigerator and set aside.By enzyme-linked immunosorbent assay experiment(ELISA)indirect legal test of serum anticardiolipin antibodies,anti-endometrial antibody,ovarian antibody,anti-sperm antibodies,anti-human chorionic gonadotropin antibodies.3 Fill out the abortion consent,the chromosomal microarray(CMA)detection of reproductive medicine of the informed consent,A routine abortion,leaving fluff organize 2x2 cm under sterile conditions,saline rinse fully into sterile containers;While extracting peripheral venous blood in patients with 2ml,citric acid sodium anticoagulation tube saved within 24 hours send along to the golden mile Test Center for testing.Results:In 25 cases analysis,1 case was failed detected because of maternal pollution and 24 cases were detected successfully;the success rate was 96.0%.There were 18 case of chromosome abnormalities,with abnormal rate 75.0%.It contains 16 cases of chromosome number abnormalities(83.3%),2 cases of chromosome structural abnormality(11.1%),1 case of chimerism(6%),5cases of villi chromosome show normal polymorphism(25.0%).Immunization of infertility test results: among the 25 cases of pediatric patients,24 cases of autoimmune antibodies in patients with negative,only one case of anticardiolipin antibodies and anti-endometrial antibodies was positive.General:(1)The villi chromosome abnormality was divided into groups of ages 23 to 29 and 29 to 35,The differences between the two groups were statistically significant(?2= 0.53,P < 0.05);(2)The initial embryonic developmental dysplasia was compared to the recurrence group villi,there was no statistical significance(?2=0.30,P>0.05);(3)Embryo dysplasia in patients in mental tension,stay up late,don't take exercise every week,the use of mobile phones and computer time > 4 hours a day,spouse,smoking,abnormal chromosome group compared with the normal group,the differences were statistically significant(P < 0.05);History of medicine before and during early pregnancy,drinking tea,coffee,take the lead in getting reproductive knowledge,mates drinking,no statistically significant difference between the two groups(P>0.05).Each of these patients had prominent characteristics in the analysis of individual etiologies.Conclusion:1 There is a higher incidence of villi in patients with dysplasia,and there is a strong need for villi;2 Mental tension,stay up late,the use of mobile phones and computer time > 4 hours a day,working pressure,never take exercise every week,spouses smoking has important influence on embryonic development the occurrence of adverse effect;3 It does not make sense to detect ACA?ASA?EMAb?AOA?resistance to HCGb antibody when looking for a cause;...