| Research content:Embryonic death is a disease seriously affect women’s physical and mental health, the increasing incidence, its etiology is very complex. Now there are a lot of relevant study of embryonic death at home and abroad. Most believe genetic, anatomical, infection, endocrine, immune, male sperm abnormalities, environmental factors, physical and chemical factors and so on result embryonic death, in addition there are quite a number of unknown etiology. Genetic factors is the most important factor, that have been recognized at home and abroad. Reported in the literature of embryonic death villi chromosome abnormality rate can reach50%～70%, therefore research on embryonic death couples and abortion villus chromosome is very important. This study probes into embryonic death couples and abortion villus chromosome examination, providing reliable laboratory evidence for researching abortion reason and genetic counseling before repregnancy, guiding eugenics.Research objective:Through this research, we can have a comprehensive understanding of chromosome factors which resulting embryonic death. Providing genetic counseling for patients who looking for embryonic death cause and guiding the healthy birth.Research methods:1. For from January2012to December2013in the center of obstetrics and gynecology hospital, we examined venous blood chromosome of92couples who want to know the causes of embryonic death. Pregnant women age≥35years old is43cases, accounting for46.7%, age<35years old is49cases, accounting for53.3%. Couples in the history of one embryonic death is40cases, accounted for43.5%,2times or more embryonic death history is52cases, accounting for56.5%.2. On the basis of92couples who voluntarily,49cases of them abortion villus have been examined chromosome. In49cases first embryonic death is23cases, accounted for46.9%,2times or more embryonic death history is26cases, accounting for53.0%. Pregnant women age≥35years old is21cases, accounting for42.8%, aged<35years old is28cases, accounting for57.1%.Research results:1. In the92couples blood chromosome examinations,5was abnormal, the abnormal rate was2.72%(5/184), in which men were2cases (1.09%), female were3cases (1.63%). Chromosome abnormal persons were all balanced translocation, of which3cases were reciprocal translocation, accounting for60%of the chromosome abnormalities (3/5),2cases were robertsonian translocation, accounting for abnormal40%(2/5).2.49cases of abortion villus cell were cultured, of which4cases were failed,45cases were successed. The success rate was91.8%. Among the45cases villi which have been successful cultured,25cases was abnormal, the abnormal rate was55.5%(25/45). Of which number abnormal chromosome were22cases, accounting for88%of the anomaly (22/25), structure abnormal chromosome were3cases, accounting for12%of the anomaly (3/25). Among the abnormal chromosome, trisomy is the largest number(16cases), accounting for72.7%of the abnormal number (16/22). Trisomy16is the most chromosome trisomy (5cases), accounting for31.3%(5/16)3.Recurrence of embryonic death and starting embryonic death group villi chromosome abnormality rate were58.3%and52.3%, two groups have no difference statistically (x2=0.161, P>0.05)4. Pregnant women(age≥35years old)which villi chromosome abnormality rate was73.6%(14/19), aged<35years group of abnormal rate was42.3%(11/26), there are statistically significant.(χ2=4.3771P<0.05)。Therefore,≥35years old pregnant women’s villi chromosome abnormality rate increased obviously.Conclusion:1. Embryonic death associated with the couple of chromosome abnormality and abortion villi chromosome abnormality.2. Women’s age≥35years old is the high risk factor which resulting villi chromosome abnormality, so the age of35or more patients examining villi chromosome is more necessary.3. The risk of villi chromosome abnormality has nothing to do with the number of embryo death.4. Chromosome examination results can guide the patients to genetic counselling before pregnancy again and guiding the healthy birth. |
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