| Objective: To investigate the expression of B-cell translocation gene 3(BTG3)in colorectal cancer and liver metastasis cancer tissues,and the effects of overexpression and silencing of BTG3 gene on proliferation,migration and invasion of colorectal cancer cells,and regulating the apoptosis of tumor cells.Combining the clinical data of patients with the liver metastasis of colorectal cancer,to investigate the correlation between BTG3 expression and clinical prognosis of patients and its clinical significance.Methods: The expression of BTG3 protein in 60 cases of colorectal cancer tissues and corresponding paracancerous tissues,liver metastasis cancer and corresponding paracancerous tissues was detected by immunohistochemical S-P method.The expression levels of BTG3 in human colorectal cancer cells were detected by RT-PCR and Western blot.The plasmids and siRNA of BTG3 gene were synthesized and transfected into human colorectal cancer cells.The transfection efficiency was detected by RT-PCR and Western blot.The inhibitory rate of cell growth was determined by CCK-8.The apoptosis rate was measured by flow cytometry.The expression levels of CyclinDl,p53 protein,apoptosis protein and EMT related protein were detected by Western blot.The relationship between the expression of Ki67 and the degree of differentiation in human colorectal cancer tissues and corresponding paracancerous tissues,liver metastasis cancer and corresponding paracancerous tissues was detected by immunohistochemical S-P method.Bivariate correlation analysis was used to analyze the correlation between the expression of BTG3 and Ki67 in the human liver metastasis of colorectal cancer.Results: It is found that BTG3 gene is low expression in colorectal cancer,liver metastasis cancer tissue and colorectal cancer cell.The silencing of colorectal cancer cells by BTG3 siRNA interference results in the following:the results of Western blot and RT-PCR show that the expression of BTG3 is decreased(P<0.05).CCK-8 assay showed that the cell growth inhibition rate was lower than that of the control group(P<0.05).The apoptosis rate of the cells was lower than that of the control group by flow cytometry(P<0.05).Western blot detected CyclinDl expression in the siRNA group were higher than the control group(P<0.05).Western blot detected p53 expression in the siRNA group were lower than the control group(P<0.05).The Western blot test showed that silencing of the BTG3 gene inhibited the development of EMT in HCT116 cells(P<0.05).When using of BTG3 plasmid DAN overexpression the colorectal cancer cells,then compares the expression level of BTG3,the result of CCK-8 test,the result of the flow cytometer test and others.With BTG3 siRNA interference silencing the colorectal cancer cells,the result is the opposite,the difference was statistically significant(P<0.05).Ki67 is highly expressed in colorectal cancer,liver metastasis cancer,adenoma and colorectal cancer metastasis lymph node,and is closely related to the differentiation degree of colorectal cancer.It is closely related to the depth of tumor invasion and lymph node metastasis(P<0.05).The expression of Ki67 in the adjacent tissues of colorectal cancer was not significantly correlated with the degree of differentiation,and there was no statistical significance(P>0.05).In addition,the expression intensity of Ki67 was related to the degree of differentiation of liver metastases,and the poorer of the differentiation,the Ki67 is higher of the expression intensity(P<0.05).At the same time,bivariate correlation analysis showed that the expression of BTG3 and Ki67 in the human liver metastasis of colorectal cancer was negatively correlated.Conclusions: BTG3 gene is abnormally low in colorectal cancer and liver metastasis tissues and colorectal cancer cells.Silencing BTG3 expression by siRNA interference and up-regulation of BTG3 expression by plasmids,they can regulate(BTG3 plays an inhibitory role when up-regulated,while BTG3 down-regulation plays a catalytic role.)the proliferation,migration,invasion,cycle regulation and EMT of colorectal cancer cells,they can induce and inhibit their apoptosis respectively.These results suggest that BTG3 may play an important role in the development and progression of the liver metastasis of colorectal cancer.At the same time,low expression of BTG3 suggests a poor prognosis in patients with the liver metastasis of colorectal cancer.BTG3 combined with Ki67 factor can predict the occurrence and development of liver metastasis of colorectal cancer,and can be used as a reference for judging the therapeutic effect of tumor. |
PDF Full Text Request