Experimental Study Of Expression Of Gene KCNJ2 Inhibited By Plasmid-Mediated RNA Interference In Rat Myocardial Cells

Background: Currently electronic pacemakers are the main therapy for heart block and sick sinus node syndromes. But it has a lot of shoutcomings:limited battery life,infections, reoperations and venous thromoembolus,etc.Furthermore,they are not suitable for patients with high risk of infection or too young.Therefore ,with the development of moleculerbiologial technology, people are trying to develop the biolog -ical pacemaker.At present, there are currently two major strategies in developing biological pacemaker:gene therapy and cell therapy. Cell therapy is definited as the use of stem cells(embryonic stem cells and mesenchymal stem cells) and sinus node cells. But there are lots of unsolved problems such as: immunne rejection, oriented differation, tumorigenicity and ethinic issue,etc. Developing biological pacemaker by gene therapy centered on three strategies:(1)Overexpressing the neu -rohormone receptors increasing atria electronic activity. (2)Overexpre -ssing the HCN2 in diastolic phase. (3)Exchanging 3 amino acid residua -ls in the inward rectifier potassium ionic channels to reducing superpo -larization currents. Both of the former methods can get only low beating rate by vagal stimulation to suppress the sinus rhythm.Therefore these methods are not suitable for making of biological pacemaker.Recently study demonstrates that adult myocardial cell has the latent pacing ability, but it is inhibited by inward rectify current...