The Study Of The Expressions Of CaⅢ And CaⅢ Mrna In Skeletal Muscles Of COPD Patients

Objective:To explore the expressions of carbonic anhydrase Ⅲ(CAⅢ)and its mRNA contents in the quadriceps femoris muscles and intercostal muscles of the control subjects and chronic obstructive pulmonary disease(COPD)patients with different severity classification.Methods:The quadriceps femoris muscle tissues of 75 patients who received operations in the Orthopedics department of the first affliated hospital of Kunming Medical University were collected,including 25 normal controls,24 mild COPD patients and 26 moderate COPD patients.The intercostal muscle tissues of 72 patients who received operations in the department of thoracic surgery of the first affliated hospital of Kunming Medical University were collected,including 23 normal controls,24 mild COPD patients and 25 moderate COPD patients.A total of 147 samples were divided into 2 groups:quadriceps femoris muscle group(Q group)and intercostal muscle group(C group).Each group was then divided into 3 subgroups based on the different severity classification of COPD:control group(FEV1/FVC>70%,FEV1%(FEV1 predicted)≥80%),I group(FEV1/FVC<70%,FEV1%(FEV1 predicted)≥80%)and Ⅱ group(FEV1/FVC<70%,30%≤FEV1%(FEV1 predicted)<80%).The CAⅢ and CAⅢ mRNA were measured using ELISA and RT-PCR and compared between different groups.Results:There is no significant difference between the Q control group and the C control group in the concentration of both CAⅢ and CAⅢ mRNA.Statistical differences were found when the comparisons of the concentration of CAⅢ carried out between the Q I and C I groups(12.10±1.10,11.03±0.90;t =3.69,P<0.001)and the QⅡI and CⅡ groups(15.40±1.05,13.52±0.91;t=6.85,P<0.001).For the concentration of CAⅢ mRNA(2-△△Ct),no significant difference was found between Q I and C I groups(2.18±0.95,2.10±0.84;t =0.305,P =0.76),but there was difference between the QⅡI and CⅡ groups(3.79±1.03,3.01±0.82;t =2.96,P=0.005).For subgroup analysis within the Q group,the CAⅢ(ng/ml)and CAⅢmRNA(2-△△Ct)were significant different among the Q control,Q I and QⅡ group(CAⅢ(ng/ml):10.13±1.22,12.10±1.10,15.40±1.05;F=143.85,P<0.001);CAⅢ mRNA(2-△△Ct):1.00±0.44.2.18±0.95.3.79± 1.03;F=69.88,P<0.001).The subgroup analysis within the C group showed that the CAⅢ and CAⅢ mRNA were also significant different among the C control,C I and CⅡ group(CAⅢ(ng/ml):10.09±0.88.11.03±0.90.13.52±0.91;F=94.58,P<0.001);CAⅢ mRNA(2-△△C):1.00±0.43.2.10±0.84.3.01±0.82;F=45.89,P<0.001).Conclusion:CAⅢ and CAⅢ mRNA concentration in the skeletal muscles of COPD patients are positively related and COPD patients got a higher CAⅢ and CAⅢmRNA concentration in some of the skeletal muscles,especially skeletal muscles of the lower limbs.For COPD patients at different stages,the CAⅢ and CAⅢI mRNA concentration differed with the severity classification.The expression of CAIII and the transcription of CAⅢ mRNA are influenced not only by the systemic factors,but also by local factors.