Clinical Study On The Function Of Mitochondrial Function Of Intercostal Muscles In Patients With Chronic Obstruction Pulmonary Disease

Objective: To study the mitochondrial function of COPD patients with different nutrition status.Methods: Twenty patients with COPD and 10 health subjects were enrolled in this study,and the COPD patients were divided into low BMI group(BMI<18.5)and normal BMI group(18.5<BMI<24.99).The health subjects were used as normal control group.The severity of the three groups was evaluated through the measurement of pulmonary ventilation function,blood gas analysis.The basic situation of intercostal muscle of the three groups was evaluated via the measurement of levels of IL-6,IL-8,TNF-α.The mitochondrial function of the intercostal muscle was evaluated via the measurement of mitochondrial protein concentration,the activities of respiratory chain complexase I-IV and SOD,the kinetics of mitochondrial permeability transition pore(MPTP)and the observation of mitochondrial morphology with electron microscopy.Analysis the correlation relationship of FEV1 with the indexes of inflammation and mitochondrial function with Pearson.Results: Compared with those of the normal group,the indexes of pulmonary function,such as FVC%,VC%,FEV1%,FEV1/predictive value,FEV1/FVC and DLco were significant decreased in COPD patients(P < 0.05).And the values of FEV1/predictive,FEV1/FVC and DLco of the low BMI group were significantly low compared with those of the normal BMI group(P<0.05).The results of blood gas analysis showed that the values of p H and paCO2 of the normal BMI group showed no significant difference compared those of the health group(P>0.05).The pH value of the low BMI group was significantly lower than those of the health group and the normal BMI groups(P<0.05),and the value of paCO2 was significantly higher than that of the normal BMI group(P<0.05).The value of paO2 of the COPD patients was significantly lower than that of the health group(P<0.05)and there was significant difference between the low BMI group and the normal BMI group(P<0.05).The indexes in intercostal muscle showed that the levels of IL-6,IL-8 and TNF-α of COPD patients were significantly higher than those of the normal group(P<0.05),and those of the low BMI group was significantly lower compared with the normal BMI group(P<0.05).The morphology of intercostal muscle showed that patients with COPD showed increased spacing between the cells of intercostal muscles and cell deformation with varying degrees,and those in patients with low BMI were more serious.Compared with the health group,the mitochondrial protein content,the activities of respiratory chain complexase I-IV and SOD enzyme and the absorption of MPTP of the COPD patients decreased significantly(P<0.05),and there was significant difference between the low BMI group and the normal BMI group(P<0.05).The morphology of mitochondrial showed that the matrix density decreased,deformation with swelling and medullary and the damage of mitrochondrial membrane and lysis.Which means different degrees of function disorder,and patients with low BMI were more serious.There was a negative correlation between FEV1 and IL-6,IL-8 and TNF-α,and had a positive correlation with mitochondrial protein content,mitochondrial respiratory chain complex enzyme I-IV and SOD.Conclusion: COPD patients have different degrees of inflammatory response and ventilation dysfunction,causing blood acid-base imbalance,hypoxia and increased carbon dioxide level,as well as mitochondrial dysfunction.With the deterioration of nutritional statue of the COPD patients,the inflammatory response,pulmonary function,the condition gradually increased compared with with normal BMI and the mitochondrial dysfunction more serious.