Impacts Of Periodontal Infection On B-la Cells In Periodontium And Spleen In Diet-induced Obesity Mice

Animal studies have shown that B-la cells can protect against the intrinsic immune mechanisms of infectious or chronic inflammatory diseases,which can quickly remove infection from the early stages of viral or bacterial invasion.Periodontitis and obesity,can cause local infection inflammation and chronic inflammation of the body,the two associated with epidemiological reports a lot,but the immune mechanism between them is still unknown.In this study,we established a model of obese compound periodontitis,based on the presence of periodontal lesions in B-la cells and the theoretical basis of obesity associated with periodontitis,explore the pathogenesis of B-la cells in both inflammatory and periodontitis states.Section 1.Impacts of periodontal infection on expression of B-la cells in gingival in diet-induced obesity mice1.ObjectivesThis chapter mainly discusses the Impacts of periodontal infection on expression and function of B-la cells in periodontium in diet-induced obesity mice.To investigate the changes of protein and mRNA expression of B-la cell membrane-specific protein CD5 and its secretion of anti-collagen I antibody(anti-COL I antibody)and Interleukin-10(IL-10)inflammatory cytokines in the periodontium of mice obese with periodontal infection.2.MethodsAnimals:Twenty-four mice were randomly divided into two groups(n = 12 per group).One group was fed with high-fat diet(HF)and the other group fed with low-fat diet(LF).After 30 weeks of diet-induced,6 mice were randomly selected from the HF group and the LF group,respectively.The 12 mice was ligated at the bilateral maxillary second molars with Pg-adhered ligature(5-0 silk)for 5 days to induce periodontitis.As the control,each group remaning 6 mice was sham ligated with sterile silk that was removed at once.Normal weight control group(LFC group),Periodontitis group(LFP group),Obese group(HFC group),obese with ligated group(HFP group).Quantitative analysis of immunohistochemical staining and real-time quantitative PCR gene detection:The levels of CD5,anti-COL I antibody and IL-10 protein and mRNA in periodontium were quantitatively detected by real-time quantitative PCR and immunohistochemical staining.The correlation between the expression level of B-la cells in periodontium and the number of inflammatory cells infiltrated in periodontium and body weight,respectively:The mice were weighed;The infiltration of inflammatory cells was quantified by HE staining of periodontium;Correlation analysis was made between the body weight,the number of inflammatory cells and the expression level of B-la cells.3.ResultsExpression of mRNA and protein in B-la cells of periodontium:The main effect of periodontitis on the three proteins of B-la cells was significant,The mRNA and protein levels of IL-10,CD5 and anti-COL I antibodies in periodontitis group were significantly higher than those in control group(P<0.001).Effects of obesity and periodontitis on the expression of B-la cells in periodontium:There was a significant positive correlation between the protein expression level of CD5,IL-10 and anti-COL I antibody and the number of inflammatory cells(r>0,P<0.001);But there was no significant correlation between the three proteins and body weight(P>0.05).4.Conclusions.B-la cells were up-regulated at the early stage of periodontitis,anti-COLI antibody can resist the early destruction of periodontal tissue.But the inflammation induced by obesity did not have a significant effect on the expression of B-1a cells.Section 2.Impacts of periodontal infection on expression of B-1a cells in spleen in diet-induced obesity mice1.ObjectivesThis chapter mainly discusses the Impacts of periodontal infection on expression of B-1a cells in spleen in diet-induced obesity mice.To investigate the changes of protein expression of B-1a cell membrane-specific protein CD5 and its secretion of anti-COL I antibody and IL-10 inflammatory cytokines in the spleen tissue of mice obese with periodontal infection.2.MethodsQuantitative analysis of protein:The protein expression levels of CD5,IL-10 and anti-COL I antibody in spleen tissue were quantitatively detected by Western blotting.The correlation between the expression level of B-1a cells in spleen tissue and the number of inflammatory cells infiltrated in gingival tissue and body weight,respectively.3.ResultsExpression of B-1a cells in spleen tissue:The main effects of obesity on the three proteins of B-1a cells were significant.The protein expression levels of CD5,IL-10 and anti-COL I antibodies in the obese group were significantly higher than those in the normal body weight group(P<0.001)Effects of obesity and periodontitis on the expression of B-1a cells in spleen tissue:There was a significant positive correlation between the protein expression level of CD5,IL-10 and anti-COL I antibody and body weight(r>0,P<0.001);But there was no significant correlation between the three proteins and the number of inflammatory cells in gingival(P>0.05).4.ConclusionsB-1a cells are up-regulated in obese cells,but their function is not clear.However,periodontal infection did not significantly affect the expression of B-1a cells in spleen tissue in obese body.