Study Of The Inhibition Of Anti-Gingipains Adhesin Domains Hgp44 Yolk Antibody(IgY) For Porphyromonas Gingivalis Induced Experimental Periodontitis In Rats

Objective Observe the inhibition of ant-gingipains adhesion domains Hgp44 yolk antibody(Ig Y) for experimental periodontitis by animal experiment.Method A total of 24 male SD rats were divided randomly into four groups, including the blank group(A), incubation with normal saline(NS) group(B), incubation with anti-Hgp44-Ig Y group(C) and incubation with hexadecylpyridinium chloride group(D).Ligation with filament was performed with four first molars in rats,then performed with processed porphyromonas gingivalis. Water with sucrose was offered. Gingiva Index(GI) and BANA test of subgingival bacterial plaque were tested 4 weeks later. After executing tested rats, alveolar bone loss(ABL) test and histological observation on gingiva were performed; while real-time quantified PCR technology was adopted to determine the relative expression levels of m RNA in IL-10, OPG and RANKL in gingival tissues of rats and calculated OPG/RANKL ratio.Result Compared with Group B, GI, ABL and BANA results are significantly lower in both Group C and Group D(p<0.01); while m RNA expression levels of IL-10, OPG and OPG/RANKLratio in gingival tissue were significantly increased(p<0.01) and the level of RANKL was significantly decreased(p<0.05)in the two groups. The differences of GI, ABL, BANA results and OPG/RANKL ratio between Group C and Group D showed no statistical significance(p>0.05);the difference of the m RNA expression levels of IL-10 and RANKL between the two groups was not significant(p>0.05);while the m RNA expression level of OPG in the Group C was significantly lower than Group D(p<0.05).Conclusion anti Hgp44-Ig Y can reduce gingival inflammation state, effectively inhibit the alveolar bone loss, slow the development process of the experimental periodontitis in rats.