Study On The Role Of LncRNA RP4-612B15.3 In The Development And Progression Of Colorectal

Objective The effect of lnc RNARP4-612B15.3 on the development of colorectal cancer was explored by the construction of lnc RNA RP4-612B15.3 lentivirus plasmid and its effect on SW-480 cell line.Methods The expression of lnc RNARP4-612B15.3 was induced by transfection of lnc RNARP4-612B15 and the expression of lnc RNARP4-612B15.3 was observed and the expression of lnc RNARP4-612B15.3 was up-regulated.The proliferation of sw480 cells was observed by overexpressing lnc RNARP4-612B15.3;And to explore the role of lnc RNARP4-612B15.3 in the development of colorectal cancer.Results The number of fluorescent cells decreased with the increase of dilution factor by fluorescence fiber microscope.The results of lentivirus titer detection were: lv3-sh-lncrna rp4-612 b 15.3 was 8x108tu/ml;lv5-nc 3x108tu/ml;lncrna rp4-612b15.3 was 4x108tu/ml;lv3-nc 5x108tu/ml.Flow cytometry sh-lnc RNARP4-612B15 positive rate was 99.8%;the positive rate of lnc RNARP4-612B15 was 96.3%;cell infection efficiency is greater than 70%,suggesting that the interference expression vector lnc RNARP4-612B15 lentivirus infected with lentivirus(lv-sh-lnc RNARP4-612B15.3)and qualified production.With the upregulation of lncrnarp4-612b15.3 also decreased expression in the colorectal cancer cell SW-480.The lentivirus infected SW-480 cells was detected to construct a stable over expression of lncrnarp4-612b15.3 we found that as the lncrnarp4-612b15.3 concentration increases,the number of virus cell lines decreased and apoptosis rate increased,the two negative correlation.So we speculate that lncrnarp4-612b15.3 may be through the lower expression level,increase the impact of the entire genome instability,thereby promoting the occurrence and development of colorectal cancer related protein detected by.Western expression in a certain sense,over expression of lncrna rp4-612b15.3 can significantly improve the expression level of Caspase3,there was a positive correlation between.After lncrna rp4-612b15.3 interference(sh-lnc-rp4)significantly reduce the expression level of Caspase3.Conclusion 1.Lnc RNARP4-612B15.3 virus plasmid(lv-sh-lnc RNARP4-612B15.3)was successfully constructed;and can effectively infected SW-480 cells.2.Lnc RNARP4-612B15.3can promote the apoptosis of colorectal cancer cell SW-480;3.As the Lnc RNARP4-612B15.3 concentration increases,the number of virus cells decreased and the apoptotic rate of increase,which is inversely related to.Lnc RNARP4-612B15.3 could decrease its expression level increased,affecting the entire genome the instability thus promotes colorectal cancer occurrence and development role in a certain sense.4.The high expression of Lnc RNARP4-612B15.3 can significantly improve the expression level of Caspase3,there was a positive correlation between Lnc RNARP4-612B15.3 after interference(sh-lnc-rp4)significantly reduced the expression level of Caspase3;resulting in colorectal cancer is through down-regulation of Lnc RNARP4-612B15.3 reduced Caspase3 activation The pathway can inhibit the apoptosis of tumor cells.