The Mechanism Of LncRNA-SNHG15 Promoting Cell Proliferation In Colorectal Cancer

Objective The aim of of this study was to evaluate the effects and potential mechanism of small nucleolar RNA host gene 15(SNHG15)on the proliferation in colorectal cancer(CRC).Methods The expression of SNHG15 was detected by real time quantitative reverse transcription PCR(qRT-PCR)in 113 paired CRC tissues.The Kaplan-Meier method and log-rank test were applied to compare the survival distribution between different groups.CCK-8 assay and colony formation assay were used to measure the effect of SNGH15 on cell proliferation.Flow cytometric analysis was performed to measure the effect of SNHG15 on cell apoptosis.The effect of SNHG15 on tumor growth was studied in animal experiments.Dual luciferase reporter gene assay was used to determine potential regulation of miR-338-3p on SNHG15 and other downstream targets.Protein expression was analyzed by Western Blot.Result 1.Our results showed that SNHG15 was up-regulated more than 1.5-fold in64.6%(73/113)of CRC tissues compared with paired NCTs(P<0.0001).High level of SNHG15 expression predicted poor prognosis of CRC(P=0.0051).2.SNHG15over-expression could promote cell proliferation and inhibit cell apoptosis.3.Animal experiments showed that up-regulated of SNHG15 promoted tumor growth in vivo.4.SNHG15 could bind to miR-338-3p and block its inhibition on FOS(RAB14),regulating cell proliferation and apoptosis.Conclusion 1.SNHG15 plays the role of oncogene in colorectal cancer.2.We reveal the potential regulation between SNHG15 and miR-338-3p for the first time.3.We identify the SNHG15/ miR-338-3p /FOS(RAB14)axis regulates colorectal cancer cell function.