The Significance Of Gastric Mucosa Glandular Lumens Roundness In The Differential Diagnosis Of Well-differentiated Gastric Adenocarcinoma

Objective The value of roundness,long diameter and short diameter of normal gastric mucosa,hyperplastic gastric mucosa and well differentiated gastric adenocarcinoma were measured by computer image analysis software.The value of roundness,long diameter and short diameter of glandular lumens were measured.To investigate the reference value of roundness,long diameter and short diameter of gastric mucosa glandular lumens in the diagnosis of well differentiated gastric adenocarcinoma.Methods We selected 30 cases of conventional HE sections pyloric well differentiated gastric adenocarcinoma received by 3 experienced pathologists consistent with a diagnosis as the study object,and the other 20 cases of gastric hyperplasia and 10 cases of normal gastric mucosa were used as control.All sections were from our pathology department of the hospital from January 2016 to July 2016,and it was scanned into digital slices at high resolution.The roundness,long diameter and short diameter were measured by image measurement software,aparting from the obvious longitudinal and oblique gland.The principle of roundness measurement is to divide the glandular region by using the threshold segmentation method in image segmentation,and to track the contour of the glandular lumen.The roundness of the contour is calculated by mathematical formula.Glandular lumens roundness were measured five times and averaged.There were 800 well-differentiated adenocarcinoma glandular lumens,500 hyperplasia glandular lumens and 300 normal glandular lumens.Roundness value and length diameter and short diameter differences were observed and analysed among the three groups and between each pair.Kruskal-Wallis rank sum test was used for analysis of the relationship among three groups.Results1.The median value of roundness of normal gastric mucosa glandular lumens was 79.7,which was greater than the median value of roundness 72.6of hyperplasia glandular lumens,and which was higher than the median value of roundness 61.6 of well differentiated adenocarcinoma.Statistically significant differences in roundness for normal mucosa,hyperplasia and well-differentiated adenocarcinoma were found(P<0.01).And roundness value of the pairwise difference among the three groups had statistical significance(P < 0.01).2.The value of long diameter of normal gastric mucosa glandular lumens was 24.1?m,which was less than the value of long diameter 46.0?m of hyperplasia glandular lumens,and which was less than the value of long diameter 114.8?m of well differentiated adenocarcinoma.Statistically significant differences in long diameter for normal mucosa,hyperplasia and well-differentiated adenocarcinoma were found(P<0.01).And long diameter value of the pairwise difference among the three groups had statistical significance(P < 0.01).3.The value of short diameter of normal gastric mucosa glandular lumens was 16.0?m,which was less than the value of short diameter 23.9?m of hyperplasia glandular lumens,and which was less than the value of short diameter 41.9?m of well differentiated adenocarcinoma.Statistically significant differences in short diameter for normal mucosa,hyperplasia and well-differentiated adenocarcinoma were found(P<0.01).And short diameter value of the pairwise difference among the three groups had statistical significance(P < 0.01).Conclusions There were statistically significant differences in the roundness,long diameter and short diameter of the pyloric gland of gastric adenocarcinoma,the gastric hyperplasia gland and the normal gastric mucosa gland.The roundness was expected to be the stomach differential diagnosis of adenocarcinoma of the pathological differential diagnosis of valuable reference indicators.