Research On Optimization Of Extraction Separation And Detection Of Pharmaceutical Chemical With Orthogonal Method

Objective: To optimize the inverse phase membrane extraction of nicotine from tobacco leaf and Oxidation of sophora alopecuroides fruit tree alkali extraction process.And laser light sources and xenon lamp light source determination of rhodamine B,vitamin B2,fluorescein and fluorescein isothiocyanate content,and to compare the fluorescence intensity of the two light source.Under acid condition,Study on laser online fluorescence spectroscopy of diclofenac potassium on Luo DanmingB fluorescence quenching effect and discuss the optimization conditions.Use statistical methods to compare three areas in xinjiang vine bleeding SAP antioxidant capacity.Method: Taking the extraction rate of nicotine as an index,the optimal process was investigated by 5 factors: acid concentration,alkali concentration,cycle time,acid and alkali aqueous phase and organic phase volume ratio and flow rate,Select the best process.With beans as raw material,the L16(45)on the basis of single factor experiment,By orthogonal test to investigate concentration of hydrochloric acid,sodium hydroxide concentration,extracting time and velocity's influence on the oxidation of fruit tree alkali extraction process,Determine the optimal extraction process.First by using laser and xenon lamp light source online fluorescence spectrometry determination of rhodamine B,vitamin B2,fluorescein and fluorescence intensity of fluorescein isothiocyanate,In order to compare the fluorescence intensity from a laser source and the xenon lamp,Then use laser excitation wavelength for 554 nm,576nm wavelength for launch,measurement of diclofenac potassium of rhodamine B fluorescence quenching effect,the difference between the blank solution and test solution fluorescence intensity ?F=F1-F0,According to the ?F value and solution of diclofenac potassium concentration was positively than relations,set up a kind of fluorescence quenching new method for determination of diclofenac potassium.On the basis of single factor experiment,orthogonal test L9(33)diclofenac potassium sulfate,rhodamine concentration and pH value on the influence of rhodamine B fluorescence quenching process,To determine the best fluorescence quenching process.Using statistical analysis methods to analyze xinjiang vine bleeding SAP of · OH,O2-and DPPH ·,NO2-,ABTS · the five kinds of free radical clearance.Using SPSS 22.0 software of data for statistical analysis.Results: the optimum extraction conditions for the extraction of nicotine in tobacco leaves: acid concentration 0.01%,alkali concentration was 5%,the cycle time of 25 min,the volume ratio(pH phase: organic phase)2.5?1,a flow rate of 6mL/min,the extraction rate of nicotine reached 99.07%.the orthogonal experiment data statistical analysis,P<0.05 show significant difference.According to the best process,3 times repeated experiments,the results showed that nicotine extraction rate reached 99.01%.Sophora alopecuroides oxidation in fruit tree alkali extraction process conditions as follows: 0.3%hydrochloric acid concentration,sodium hydroxide concentration is 0.75mol/L,60 min cycle time,volume ratio 1?1,the velocity of 6mL/min.The results of variance analysis showed that there were significant differences between the other factors except the flow rate(P<0.05).There was a significant difference between the fluorescence intensity of the laser light source and xenon lamp(P<0.05).Variance analysis found that three factors of rhodamine B fluorescence quenching value,the influence of the delta F exist significant difference(P<0.05),the best fluorescence quenching process of diclofenac potassium 1.0x10-4mol/L,rhodamine concentration of 1.2x10-6mol/L,pH value of 2.5.Single factor variance analysis results show that Khartoum nagel,turpan seedless white and hotan sand according to buck township red grape vine injury flow ability of five kinds of free radical removal has significant difference(P<0.05),Multiple comparison,known to hydroxyl free radical removal ability and other four kinds of free radical removal ability has significant difference(P<0.05),Three regional area vine bleeding SAP 5 kinds of free radical clearance ability between there is no difference(P>0.05).Conclusion: The operation is simple,fast extraction time,the consumption of organic solvent,easy to realize industrialization,environmental pollution,less applicable to the nicotine in tobacco leaves and oxidation in fruit tree alkali extraction of sophora alopecuroides,has a certain theoretical foundation for application.And Oxysophocarpine comprehensive development,using a certain foundation.The laser light source is widely used in the field of drug determination and fluorescence quenching.There was no significant difference between the 3 regions in grape SAP bleeding.However,the scavenging ability of OH free radical was stronger than that of other free radicals,and there was a significant difference.