The Significance Of Th1/Th2/Th17 Cytokines In The Serum Of Patients With Pulmonary Tuberculosis

Objective : To evaluate the significance of cytometric beads array(CBA)in the detection of Th1/Th2/Th17 cytokine in patients with pulmonary tuberculosis.Methods:The levels of 7 cytokines(IL-2,IL-4,IL-6,IL-10,TNF,IFN-γ and IL-17A)were detected by CBA according to the instruction of BD CBA Human Th1/Th2/Th17 Cytokine Kit(BD Biosciences,USA)in the serum of 98 patients with tuberculosis(38 sputum-smear positive and 60 sputum-smear negative)and 79 healthy individuals.Then analyze the relationship and differences among these groups.Results:Except IL-17 A,the level of IL-2,IL-4,IL-6,IL-10,TNF,IFN-γ was higher in the patients group than control group;As to IL-2,IL-4,IL-10 and IFN-γ,the sputum-smear positive group had a higher level than control group,but the sputum-smear positive group had no difference with sputum-smear negative group.Compared with sputum-smear negative group,the sputum-smear positive group have a higher level of IL-6 and TNF.Conclusion:IL-6 and TNF can be used to monitor the prognosis of tuberculosis.Meanwhile,CBA is sensitive,could detect several cytokines once with less sample consumption and time,thus could be applied for the monitor of tuberculosis progress.