Study On Analytic Capability Of Flow Cytometric Bead Array For Lung Cancer Serum Makers

This study aim at appraising analytical capability of flow cytometric bead array for lung cancer markers through the tests of limit of detection,relative standard deviation,specificity,methods comparation and linearity rang.This study report the limit of detection,relative standard deviation,specificity and linearity rang in flow cytometric bead array for serum carcinoembryonic antigen(CEA),cytokeratin 19(Cyfra21-1)and neuron specific enolase(NSE).Western blotting method was ultilized to validate the specificity of antibody-antigen recognization.By adding three acyl glycerol,hemoglobin and bilirubin,the interference test was conducted.Compared to electrochemiluminescence immunoassay,the relative error for flow cytometric bead array was assessed.Flow cytometric bead array demonstrated that the limit of detection was 1.71 pg/mL for CEA,3.97 pg/mL for cyfra21-1,and 2.27 pg/mL for NSE.The relative standard deviation for intra-assay and inter-assay were below 10% and 15%,respectively.The pair of antibodies can defferentially recognize antigens.The measurement for CEACAM6,CK18,NSE appeared that there was no significant cross-talking reaction.Three acyl glycerol and bilirubin did not significantly interfere with the detection for serum samples.Hemoglobin of 500 ng/mL can significantly interfere with the detection of Cyfra21-1(P < 0.05)and NSE(P < 0.05).The correlation coefficient between flow cytometric array and electrochemiluminescence immunoassay was 0.9842 for serum CEA,0.9622 for serum cyfra 21-1 and 0.982 for serum NSE.The linearity ranged from 355.76 pg/mL to 367.74 ng/mL for CEA,from 87.89 pg/mL to 107.8 ng/mL for cyfra21-1,and from 90.12 pg/mL to 86.07 ng/mL for NSE.In conclusion Flow cytometric array for lung cancer markers may be of use in clinical detection.This paper also describes the progress in study of tumor markers such as CEA,Cyfra21-1,NSE,etc.and the correlated test method.In particular,the application of flow cytometry in the fields of hematology,oncology,and immunology was introduced in detail.