Correlation Study About The Effect Of IL-33/ST2 Signal Pathway Single Nucleotide Polymorphisms On Ischemic Stroke

Study Background and ObjectivesIschemic stroke(IS)is caused by interruption of blood flow to the brain because of vascular stenosis and occlusion,deprives tissues and cells of oxygen,which lead to a series of clinical symptoms about neurologic impairment.Early control of the risk factors and evaluate prognosis of disease are important for effective care to improve the functional outcome.Interleukin-33(IL-33)is a new member of the IL-1 cytokine family which is recently discovered.IL-33 is widely expressed in multiple tissues and cells,involved in the pathogenesis of many human diseases.Nowadays,several studies has affirmed that IL-33 plays a important protective role in cardiovascular diseases.Some recent studies showed that IL-33 can be used as a novel biomarker to independently diagnose and prognose IS.Previous studies have shown that IL-33 binding to its receptors Homolog of sulfo-transferase 2(ST2)and IL-1 receptor accessory protein(IL-1RAcP)to activate a variety of immune cells and further produce Th2 cytokines to enhance Th2-type immune responses,and IL-33 tilting the Th1/Th2 balance.The biological functions can regulate the process of immunological and inflammatory reaction.This pathway is called IL-33/ST2 signal pathway.Studies have confirmed that the single nucleotide polymorphisms(SNPs)of the single locus rs11685424(the genes encoding ST2),and the single locus rs4624606(the genes encoding IL-1RAcP),are related to myocardial infarction.However,the relationship between SNPs and IS is not clear.In this study,we compared the differences of SNPs of three single locus rs11685424,rs950880,and rs4624606,which in the genes encoding IL1RL1 and IL-1RAcP between IS patients and healthy controls to explore whether there is a correlation between IS and IL-33/ST2 signal pathway SNPs.MethodsThe IS patients and healthy controls were consecutively enrolled from January 2014 to December 2015 in the 3rd department of Neurology,the First Affiliated Hospital of Zhengzhou University.The t test and ?2 test are used to compare the differences about demographic features and Total cholesterol(TCHO)level,Triglyceride(TG)level,High density lipoprotein(HDL)level,and low density lipoprotein(LDL)level between IS patients and controls.The relevance between IS and IL-33/ST2 signal pathway SNPs are calculated by Spearman correlation coefficients.ResultsAll participants include 776 IS patients and 776 age-and gender-matched controls with no cerebralvascular diseases history or cardiovascular diseases his-tory.In the patient group,there are 415 patients caused by large-artery atheros-clerosis and 361 patients caused by small-artery occlusion lacunar.There is no statistical significance between IS patient group and healthy control group about SNPs of three single locus rs11685424,rs950880,and rs4624606(P > 0.05).Similarly there is no statistical significance between large-artery atherosclerosis group and small-artery occlusion lacunar group about SNPs of three single locus rs11685424,rs950880,and rs4624606(P > 0.05).There is no statistical significance between large-artery atherosclerosis group and healthy control group about SNPs of three single locus rs11685424,rs950880,and rs4624606 as well(P > 0.05).ConclusionBased on our study,the results suggest there is no correlation between IS and IL-33/ST2 signal pathway SNPs of receptors IL1RL1 and IL-1RAcP.