| Objective: CD147,which was involved in inflammatory reaction,thrombosis and atherosclerosis of pathophysiological processes in ischemic stroke,may be a candidate gene susceptible to ischemic stroke.In the present study,a case-control study was used to investigate the expression of CD147 m RNA in peripheral blood mononuclear cells(PBMCs)and plasma protein,and CD147 3’-UTR polymorphism(rs13676A/G and rs1063376C/T)association with risk of ischemic stroke.Besides,the genotypes of 3’-UTR polymorphisms relation with the transcription and translation levels of CD147 were also analyzed,with the aim of providing molecular biological evidence for the prevention and diagnosis of ischemic stroke.Methods: Fasting peripheral blood samples were collected including482 Chinese Han patients with ischemic stroke(cases)and 402 healthy subjects(controls).The CD147 m RNA relative expression levels in PBMCs were detected by quantitative real-time polymerase chain reaction(q PCR)between cases and controls.The CD147 plasma protein was measured using enzyme linked immunosorbent assay(ELISA).Genotypes of CD147 3’-UTR polymorphism(rs13676A/G and rs1063376C/T)were determined using PCR restriction fragment length polymorphism(RFLP-PCR)and DNA sequencing analysis.Statistical analyses were performed using the SPSS for Windows software package version 13.0Results:1.The CD147 m RNA relative expression levels in patients with ischemic stroke(0.9635±0.16888)were significantly lower than those of controls(1.32367±0.15284)(P<0.001).There was no significant difference of the CD147 plasma protein levels between cases(132.7794 ± 7.90702)and controls(143.5505 ± 10.94023)(P=0.27).2.The genotypes distribution of the CD147 3’-UTR polymorphisms in controls was in agreement with that expected under HWE(rs13676A/G:P=0.80;rs1063376C/T: P=0.18),indicating the controls were representative.The allele G frequencies of rs13676A/G polymorphism betweeen cases and controls were 98.13% and 98.63%,respectively.Any allele frequency of rs13676A/G polymorphism showed no significant difference betweeen cases and controls(P=0.411).Only genotype AG and GG of rs13676A/G polymorphism were detected betweeen cases and controls.The genotype of rs13676A/G polymorphism was also not statistically significant(P=0.407).The allele T frequencies of rs1063376 C/T polymorphism in ischemic stroke patients(8.81%)were significantly lower than those of controls(11.82%)(P=0.038,OR=0.722,95% CI=0.530-0.983).Heterozygote,homozygote,dominant and recessive genetic models were used to analyze the relationship between genotype of rs1063376C/T polymorphism and the risk of ischemic stroke.The significant difference was found between homozygote and recessive genetic models(homozygote: P=0.025,OR=0.113,95%CI=0.014-0.921;recessive: P=0.027,OR=0.117,95% CI= 0.014-0.958).The similar results were also obtained through logistic regression analysis after adjusting for variables such as age and gender.3.To evaluate the biological function of the CD147 rs1063376C/T polymorphism,we analyzed the correlation analyses between the m RNA and protein levels of CD147 and the rs1063376C/T polymorphism genotype,using the aforementioned data and genotyping data derived from 75 healthy individuals.The genotypes CC,CT and CT/TT of rs1063376C/T polymorphism did not affect CD147 m RNA or protein levels(P> 0.05).The similar results were obtained in cases.Conclusions: It indicated that the CD147 gene might play an important role in the pathophysiological process of ischemic stroke.The rs1063376C/T polymorphism in CD147 3’-UTR might be associated with ischemic stroke susceptibility in a Chinese Han population,which the allele T and genotype TT might be a protective factor.The rs13676A/G polymorphism was not associated with ischemic stroke.The genotype of rs1063376C/T polymorphism did not affect transcription and translation levels of CD147. |
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