The Effect Of PD-1 On The Phosphorylation Of PLC-? And PKC-? In PBMCs, CD3+T Cells And CD8+T Cells During HIV-1 Infection

Objective: In this study,we plan to explore the expression of PD-1(programmed death 1),Phospholipase C-?(PLC-?),Protein kinase C-?(PKC-?)in PBMCs(Peripheral blood mononuclear cells),CD3+T cells and CD8+T cells during HIV-1(human immunodeficiency virus-1)infection.Furthermore,we want to find the correlation of PD-1between the phosphorylation levels of PLC-? and PKC-?.Our aim is to show the exact mechanism that PD-1 impact on phosphorylation of PLC-? and PKC-? signal pathway of PBMCs,CD3+T cells,and CD8+T cells during HIV-1 infection.Method: 1.We collected peripheral blood of 42 HIV-1 carriers and 14 persons from the healthy control group according to the diagnostic criteria of HIV/AIDS in Chinese health industry.Then we divided all the patients into two groups including typical AIDS patients and HIV-1 carriers(n=14)by CD4 Count in peripheral blood.All patients excluded hepatitis B virus and hepatitis C virus infection.2.PBMCs were isolated by gradient centrifugation on Ficoll-Hypaque.3.All the cells were stimulated by purified anti-human CD3 and purified anti-human CD28.Then,cells are fixed and permed,marked up with monoclonal fluorescent antibodies such as CD3,CD8,PD-1,p PLC-?,p PKC-?.4.The frequencies of PD-1,p PLC-?,p PKC-? were evaluated by flow cytometry.5.We assessed the relationship between the expression of different indicators by using unpaired test.The correlation of different parameters in the same sample was detected by Spearman correlation analyze at the same time.Results: 1.Compared with HDs,HIV-1 infection resulted in a s ignificant increase in PD-1 and p PLC-? expression in PBMCs,and reduction of the p PKC-? expression.Correlation analysis showed that CD4 Count was negatively associated with PD-1 expression and positively correlated with p PKC-? in PBMCs.Furthermore,PD-1 expression was negatively associated with p PKC-?.There was no association between PD-1 and p PLC-? expression on PBMCs during HIV-1 infection.2.Compared with HDs,HIV-1 infection resulted in a significant increase in PD-1 and p PLC-? expression on CD3+T cells,and reduction of the p PKC-? expression.Correlation analysis showed that CD4 Count was negatively associated with PD-1 expression and positively correlated with p PKC-? on PBMCs.Furthermore,PD-1 expression was negatively associated with p PKC-?.There was no association between PD-1 and p PLC-? expression on CD3+T cells during HIV-1 infection.3.Compared with HDs,HIV-1 infection resulted in a significant increase in PD-1 and p PLC-? expression on CD8+T cells,and reduction of the p PKC-? expression.Correlation analysis showed that CD4 Count was negatively associated with PD-1 expression and positively correlated with on p PKC-? PBMCs.Furthermore,PD-1 expression was negatively associated with PKC-?.There was no association between PD-1 and p PLC-? expression on CD8+T cells during HIV-1 infection.Conclusion: The simulation results demonstrated PD-1 significantly increased on PBMCs,CD3+T cells and CD8+T cells is correlated with HIV-1 progression,and there is negative correlation between PKC-? and PD-1.We suggest that PD-1 is involved in the immune response via regulated phosphorylation of PKC-? during chronic HIV-1 infection.As the upstream signal molecule of PKC-?,there was no correlation between the expression of PLC-? and PD-1,suggesting that there are additional ways to influence the phosphorylation level of TCR-CD3 signaling molecule PLC-? in HIV-1 infection.