Quality Standard For Ziheche Medicinal Materials

Ziheche(Placenta Hominis)is the human placenta after processing,which is also called afterbirth,etc.Human placenta medicinal history dates back hundreds of years,and its use in the present constantly developing,ancient and modern history of using both prove that it has good curative effect in clinic.Because of this,combined with the particularity of source dried human placenta,as traditional Chinese medicine in recent years,the market price is also a rising tide lifts all boats.Many illegal sources of molecules with pigs,cattle,sheep placenta as dried human placenta,as well as fake and salt,starch,sugar,as a result of improper processing,heavy metal content is exorbitant,blood is not completely clean,and from the phenomenon such as virus maternal placenta,to the market and the impact of patients take the harsh.While the current standard only the description of the characteristics of simple,completely reach the purpose of the separate the wheat from the chaff.So it is necessary for the improvement of its quality standards to conduct a comprehensive.Through in this paper,the experimental research shows that the identification,microscopic identification and thin layer chromatography HPLC identification and sds-page were failed to show the real thing dried human placenta and common pigs,cows,sheep placenta placenta placenta falsify the difference.Real-time fluorescent quantitative PCR method and qualitative identification of dried human placenta,and USES the control medicinal materials as the accompanying controls,the results show that dried human placenta authenticity and common pigs,cows,sheep placenta placenta placenta falsify can effectively distinguish,and with good reproducibility,therefore it is included in the draft standard;Enzyme standard method were used respectively to check the hepatitis b virus(HBV),enzyme-linked immunoassay check HIV antibody and hepatitis a antibody,and the result can be the normal reaction of the dried human placenta medicinal materials are true.According to the data obtained from the experiment,the standard formulation was made for four general inspection items,such as water,total ash and heavy metal and harmful elements.In micro particle enzymatic chemiluminescence method for the ? HCG and P content of dried human placenta medicinal materials in research,according to the results of this method by? HCG levels in human placenta can be measured by time not neat,and is not stable,so the measurement is not applicable to establish standards.Comparison,using the same method for dried human placenta composition stability of progesterone in the determination of research found that when the content is relatively stable in the medicine,good reproducibility,high sensitivity.