| Inflammation doctrine" theory is quite popular nowdays,as a kind of explain the new theory of diabetes and obesity can lead to appear.A lot of different reasons can lead to inflammation reaction,AMPK SIRT1 / nf-kappa B signaling pathways closely related to energy metabolism,AMPK SIRT1 / nf-kappa B inflammatory response mediated by mainly affected by the body energy metabolism changes.AMPK can direct perception of the body’s energy metabolism changes,and then activate downstream SIRT1 molecules,SIRT1 activity will cause adverse effect to the activity of the nf-kappa B,results in the decrease of the activity of the nf-kappa B,thus improving the body oxidative stress occurs.Long-term and regular aerobic exercise such as swimming and metformin for oral administration can significantly improve the state of inflammation in the body and can effectively prevent insulin resistance to type 2 diabetes and enhance the body’s ability to resist the inflammatory state.Objective: the aim of this study is through the study of AMPK diabetic rats kidney/SIRT1 / nf-kappa B signaling pathways of each protein expression levels and renal oxidative stress and metformin for oral administration,aerobic exercise on diabetic rats inflammatory response and the influence of various protein expression is signaling pathways.Hope that the study found that these two kinds of interference factors in diabetic rat kidney signaling pathway mediated the influence of inflammatory reaction mechanism.Explore drugs and movement to the positive role of the body,to improve the type II diabetes and late kidney inflammation and reduce insulin resistance provides a new train of thought.Methods: buy eight weeks of 85 male SD rats,and randomly selected five N1 for blank group,the rats fed high-fat feed and STZ injection preparation into Ⅱ diabetes model,and then select the prompt building successful diabetic rats were randomly divided into five groups,respectively is group D(diabetes control group),O(aerobic group),M group(drug),OM(aerobic comprehensive intervention group),10 in each group.Aerobic group movement scheme of concrete for all O group adaptability training a week in rats,keep them to the swimming suit,and then in the formal experiment swimming six times a week,every time lasted one hour,take a day off every week.Motion plan time executed in rats after the break,immediately remove kidney tissue processing and preservation,late determination of all kinds of needed data,then analysis and comparison,the difference between the different groups come to the conclusion.Results: 1,O,M,OM,group D at the end of the experimental rats weight appeared different degree of decline,but O,M,OM of three groups of rats with group D weights are no significant difference(P > 0.05),show that aerobic exercise and drug intervention on changes have no direct effect on body weight in rats.2,O,M,OM rats of three groups of factors on the intervention there is an obvious decline blood sugar levels,which O and M group blood sugar levels compared withgroup D drop has very significant difference(P < 0.01),compared with group D OM group reduced blood sugar had significant difference(P < 0.05),the MDA content of 3,O group than group D there was very significant lower(P < 0.01),the MDA content of M group and OM group also has a significantly lower(P < 0.05).Compared with group D(156.31),and N group(236.43)T-SOD values can be found that diabetes can significantly reduce T-SOD activity in the kidney tissues.Aerobic exercise can significantly increase T-SOD activity in the kidney tissues,contrast group D(156.31)and O group(201.87).Compared to group M(173.41)and group D(156.31)after found that metformin to increase kidney T-SOD activity has certain help,but far from aerobic exercise to bring the role of effective.Indicates that the aerobic exercise and metformin to improve levels of oxidative stress of rats can bring different degree of improvement.4,D group in the rat kidney tissue relative to other groups,there is an obvious pathological changes of rats.5,O set of AMPK and compared the nf-kappa B protein expression quantity has a significant change in group D,AMPK expression significantly increases,the nf-kappa B protein expression significantly lower,M AMPK expression compared with D group has significantly increased,but for the nf-kappa B decreases no apparent protein expression.Under the condition of two kinds of comprehensive intervention intervention factors,OM group of two kinds of protein expression quantity while compared with group D also have positive changes,but not as good as the single aerobic group improved significantly.Conclusion: 1,aerobic exercise and metformin can control and treatment of type 2 diabetes bring positive effect,the specific performance in can control and improve the physiological indicators,reduce oxidative stress levels in the body 2,for a long time under a state of diabetes rats,promote the expression of inflammatory factor increases in the kidney,anti inflammatory cytokines expression decreased,at the same time AMPK SIRT1 / nf-kappa B inflammatory signaling pathways suppressed.Long-term aerobic exercise(swimming)can reduce the expression of inflammatory factors in diabetic rat kidney,at the same time promote the expression of anti inflammatory cytokines.This process may be by influencing the AMPK SIRT1 / nf-kappa B signaling pathway.3,the body’s aerobic exercise capacity may be limited by the effect of metformin,the reason may be that metformin to produce more lactic acid and lower the body’s blood lactic acid removal ability,leading to a significant rise in blood lactic acid concentration in the body.To increase the body in the sports fatigue,reduce the aerobic exercise capacity. |
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