The Inhibit Effects Of 18?-Glycyrrhetinic Acid On Gastric Tumors In K19-C2mE Transgenic Mice

Objective: After give 18?-glycyrrhetinic acid for transgenic mice,explore the effectiveness on gastric cancer in general morphology,the expression of the inflammatory response and inflammatory,inhibited the development and progression of gastric tumor in vivo,and suppressed cell proliferation and cell cycle progression in vitro,thereby inhibiting gastric cancer development.Explore the chemical prevention of 18?-GRA on transgenic mice and provide a novel drug for gastric cancer chemopreventio.Methods: The K19-C2 m E transgenic mice were used to establish the gastric mass model,and were divided into 18?-GRA-treated group and control group.The mice in the experimental group were treated with 18?-GRA and the mice in the control group were fed normally.After 52 weeks,the two groups of were sacrificed.The effects of 18?-GRA on the body weight curve,the incidence of gastric cancer,the gross morphology of gastric tissue,the HE staining,the immunohistochemical results and the gastric cancer cell lines were observed.And then analyzed the effects of 18?-GRA on gastric neoplasms.Results: There was no significant difference in body weight between 18?-GRA mice and control mice.In the control group,the gastric mucosa of the transgenic mice showed obvious tumor and infiltration,and18?-GRA-treated group were significantly less than the control group.At the same time,the tumor in the 18?-GRA-treated group was significantly smaller than that in the control group.In the control group,the expression of COX-2,Wnt-1,?-catenin ptotein in the 18?-GRA-treated group were significantly lower than those in the control group.By Annexin V method,18?-GRA treatment inhibited the development and progression of gastric tumor in vivo,and suppressed cell proliferation and cell cycle progression in vitro.Conclusion: 18?-GRA can significantly reduce the inflammation of gastric tissue in K19-C2 m E transgenic mice,reduce the incidence of gastric cancer and tumor burden,inhibited the development and progression of gastric tumor in vivo and suppressed cell proliferation and cell cycle progression in vitro,and reduce the expression of COX-2,Wnt-1 and ?-catenin proteins in gastric mucosa.