Research On The Protective Effects Of APS Against Adverse Effects Produced By ?-CP On Liver Of Mice

Objective:To study the acute toxic effects of ?-CP on liver in mice from three aspects: cell oxidative damage,protein synthesis and energy metabolism.In order to provide theoretical basis for workers to take protective measures who will contact ?-CP in workplace.And to explore the protective effects of APS on ?-CP induced acute liver injury in mice.In order to provide experimental data and scientific basis for developing effective antidote of acute synthetic pyrethroids pesticide poisoning.Methods:60 ICR male mice of clean grade were randomly divided into five groups after adaptive feeding 1 week,including the control group?corn oil?,the experimental group??-CP 40 mg/kg?and the protection groups??-CP 40mg/kg with APS 100mg/kg,200mg/kg,300mg/kg respectively?,12 in each group.The mice were treated with the reagents through gavage daily for 10 consecutive days.Observing and recording the changes of mice in fur and weight,mental state,behavior activity,drinking and feeding along the exposure process.Afterwards,biomarkers including the AST,ALT,AKP,TP,ALB,GLO in serum and SOD,CAT,LDH,ATPase,MDA in liver tissue were determined,A/G and liver organ coefficient were calculated,and pathological alteration were observed.Statistical analysis of the data were measured by statistical software SPSS20.0,and the experimental results were shown as ???±s.One-way analysis of variance,q test,and paired t test were used in comparing between multiple groups,between two groups,and within the group respectively.The difference was statistically significant when P < 0.05.Results:1.Effects of APS on general condition in ?-CP exposured miceMice in ?-CP exposure group appeared whole body poison symptoms,and which had been improved in protection groups.The weight of mice in all groups showed no significant differences?P>0.05?.2.Effects of APS on liver weight and organ coefficient in ?-CP exposured miceCompared with control group,the liver organ coefficient in ?-CP exposure group was increased,and compared with ?-CP exposure group,the liver organ coefficient in all protection groups were decreased,and all of them showed no significant differences?P>0.05?.3.Effects of APS on Acute oxidative liver damage in ?-CP exposured miceCompared with control group,the activities of serum AST,ALT and AKP in ?-CP exposure group were increased,and compared with ?-CP exposure group,the activities of serum AST,ALT and AKP in all protection groups were decreased,and all of them showed significant differences?P <0.05?.Compared with control group,the liver tissue SOD and CAT activities were decreased,MDA content was increased in ?-CP exposure group,and compared with ?-CP exposure group,activities of SOD in all protection groups and of CAT in 200 mg/kg dose protection group were increased,contents of MDA in all protection groups were decreased,and all of them showed significant differences?P <0.05?.4.Effects of APS on liver function of protein synthesis in ?-CP exposured miceCompared with control group,the contents of serum TP and ALB in ?-CP exposure group were decreased,and compared with ?-CP exposure group,the contents of serum ALB in all protection groups and of serum TP in 200 mg/kg and 300 mg/kg dose protection groups were increased,and all of them showed significant differences?P <0.05?.After exposure,A/G in all groups had appeared no inversion and showed no significant differences?P>0.05?.5.Effects of APS on hepatic energy metabolism in ?-CP exposured miceCompared with control group,the liver tissue LDH,Na⁺K⁺-ATPase and Ca²⁺Mg²⁺-ATPase activities were decreased in ?-CP exposure group,and compared with ?-CP exposure group,activities of Na⁺K⁺-ATPase and Ca²⁺Mg²⁺-ATPase in all protection groups and of LDH in 200 mg/kg dose protection group were increased,and all of them showed significant differences?P <0.05?.6.Effects of APS on liver tissue morphological alterations in ?-CP exposured miceThe photos of mice liver pathological slices indicated that: compared with control group,the hepatic lobule was in poor structure,the hepatic cord and sinusoid appeared disordered arrangement,some liver cells became irregular shape,and there were massive inflammatory cell infiltration in ?-CP exposure group;and compared with ?-CP exposure group,the structure of hepatic lobule and the shape of cells were improved,the hepatic cord and sinusoid turned into ordered arrangement in protection groups.Conclusion:1.?-CP can increase the serum AST,ALT and AKP activities,decrease the liver tissue SOD and CAT activities while increase MDA content.Which indicate that ?-CP may cause oxidative liver damage in mice,destroy the integrity of the liver cell membranes,and give rise to intra-hepatic biliary obstruction.2.?-CP can decrease the contents of serum TP and ALB and the activities of liver tissue Na⁺K⁺-ATPase,Ca²⁺Mg²⁺-ATPase and LDH.Which indicate that ?-CP may reduce the liver function of protein synthesis and lead to hepatic energy metabolism disorder.3.?-CP and metabolite may bring about liver tissue pathological damage.4.APS can have antagonistic protection on ?-CP induced acute liver injury in mice.