Study On The Pharmacological Substances And Mechanism Of Ginseng Polysaccharides To Improve Liver Cell Damage Caused By Oxidative Stress

Objective:This study established a model of BRL-3A cell injury induced by H₂O₂ in vitro.Based on the damage model,the material basis screening of ginseng has a protective effect.Based on this,explore the mechanism of ginseng active ingredients protecting liver cell damage by regulating mitochondria and gluconeogenesis of liver cells.Provide data support for further research on ginseng's efficacy.Methods:1?After H₂O₂ was used to damage liver cells,cells was given three active ingredients of ginseng.Cell viability?cell density?time of injury?concentration of administration and the best active ingredient of ginseng were determined by MTT method.2.Flow cytometry was used to detect BRL-3A cell apoptosis levels,MMP changes and ROS detection in each group.3.ELISA kits were used to detect liver glycogen,ATP,SOD,MDA,G6P,PEPCK,etc.4.RT-PCR technology was used to carry out changes in target gene transcription levels in different administration groups,and Western blot technology was used to carry out changes in target protein expression levels.The potential role of ginseng polysaccharides in protective effects was studied by exogenously adding PGC-1?inhibitors.Result:1.After screening,ginseng polysaccharides are superior to ginseng protein and ginsenosides among the three active ingredients of ginseng?p<0.05?.Ginseng polysaccharide has significant recovery effect on H₂O₂ injured rat hepatocytes,and it is time-dose dependent.The most effective concentration is 1.6 mg/ml?P<0.05?.2.Assayed by flow cytometry,ginseng polysaccharide can effectively reduce the level of apoptosis.By increasing the mitochondrial membrane potential to restore H₂O₂ damage to liver cells,at the same time,reducing the increase of reactive oxygen species caused by oxidative stress has a concentration-dependent relationship.3.Compared with the injury group treated with H₂O₂ alone,ginseng polysaccharides attenuated the up-regulation of H₂O₂-induced Bax protein expression levels,At the same time,the expression of Bcl-2/Bax increased at the level of qPCR,which effectively prevented the occurrence of apoptosis?p<0.05?.4.Ginseng polysaccharide can restore liver glycogen reduction and ATP content reduction caused by oxidative stress.By increasing SOD,reducing MDA to weaken the damage caused by H₂O₂,and increasing the content of G6P and PEPCK to restore the gluconeogenesis of liver cells,the pharmacodynamic mechanism of ginseng polysaccharides was investigated.5.It was confirmed by Western blotting and qPCR technology that ginseng polysaccharide could significantly increase the expression of PGC-1??p<0.05?in a concentration-dependent manner.Ginseng polysaccharides depend on SIRT1/PGC-1?pathway to increase PGC-1?expression.After the intervention of PGC-1?inhibitor,the protein level of PGC-1?was significantly reduced?p<0.05?,and the protective effect of ginseng polysaccharide on damaged liver cells decreased.Conclusion:1.Ginseng polysaccharide has a certain protective effect on H₂O₂-induced hepatocyte injury in rats and it has a concentration-dose dependence.2.Ginseng polysaccharide restores mitochondrial function of liver cells by reducing MMP,so that mitochondria normally produce ATP,thereby increasing ATP content.3.Ginseng polysaccharides restore liver cell function by improving the gluconeogenesis of liver cells and reducing oxidative stress damage,thereby restoring liver glycogen output.4.Ginseng polysaccharides reduce the damage of liver cells by oxidative stress by activating SIRT1/PGC-1?signaling pathway and increasing the expression of PGC-1?.