Effect Of Sappan Wood Acetlc Ether Extract On IL-18?IL-10 In The Serum Of Rats Of The Atherosclerosis Model

Objective:By observing the effects of sappan wood acetic ether extract on serum IL-18 and IL-10 levels and IL-18/IL-10 in atherosclerosis model rats and the Pathological changes of abdominal aortic vessel wall.To provide an objective basis for the study of anti-atherosclerosis effect of sappan wood acetic ether extract.Methods:60 rats were randomly divided into two groups:SD rats were randomly selected and randomly selected as the blank control group.The rest of the rats were injected with vitamin D3 70 million IU/(kg�d)intraperitoneally.After 3 days of continuous injection,Week for atherosclerosis model.After the model was successfully replicated,the rats in the model control group were treated with 10mg/ml sodium carboxymethyl cellulose solution,simvastatin group and 10mg/ml simvastatin solution,equivalent to simvastatin dose 4.2mg/(kg�d),and the low,medium and high dose of the extract of hematoxylin acetate was given 10mg/ml equal volume,medium and high concentration of ethyl acetate extract of hematoxylin,which was equivalent to 0.0575mg/(kg�d)?0.115mg/(kg�d),0.230mg/(kg�d),once daily for 4 weeks.The histopathological changes of abdominal aorta were observed by HE staining.The changes of serum IL-18 and IL-10 were detected by enzyme-linked immunosorbent assay(ELISA).Results:1.Compared with the model control group,the pathological damage of the abdominal aorta was significantly reduced in the high,medium and low dose groups of simvastatin group and hematoxylin acetate extract.2.Compared with the blank group,the levels of serum IL-18 in the model group,simvastatin group and the hematoxylin acetate extract were higher than those in the blank group,the difference was statistically significant(P<0.05).There was no significant difference in the high,middle and low dose groups of simvastatin group and hematoxylin acetate extract in the model group(P<0.05),but there was no significant difference between the two groups(P>0.05).3.Compared with the model control group,serum IL-18 level and serum IL-10 level were higher in the high,middle and low dose groups of simvastatin group and hematoxylin acetate extract,the difference was statistically significant(P<0.05)4.There was no significant difference in serum IL-18 level and IL-10 level between the high,medium and low dose groups of the extract of hematoxylin,compared with the simvastatin group(P>0.05).Conclusion:1.Hematoxylin acetate extract can relieve the pathological damage of abdominal aorta vessel wall in atherosclerotic model rats.2.The level of IL-18 and IL-10 in serum of rats with atherosclerosis were decreased,so that serum IL-18/IL-10 tends to balance,to maintain the body inflammation/anti-inflammatory balance,reduce the body's inflammatory response.