The Expression And Its Significance Of Sirt1 In Glomerulus Of Children With Henoch-Sch?nlein Purpura Nephritis

ObjectiveDetection the expression of Sirt1,NF-?B p65 in glomerulus of children with Henoch–Sch?nlein purpura nephritis(HSPN),normal renal tissue and minimal change nephrotic syndrome(MCN)through immunohistochemical technology.Analysising the correlation between Sirt1 and NF-?B p65 which expressed in glomerulus tissue.And then exploring the role and significance of Sirt1/NF-?B p65 pathway in the development of Henoch–Sch?nlein purpura nephritis.To investigate the protective effect of Sirt1 in glomerulus of children with HSPN,by exploring the correlation between Sirt1 and the renal pathological grade,IgA deposition,urinary protein level,serum creatinine and blood urea nitrogen in children with purpura nephritis.Methods45 cases(n = 45)of children with Henoch-Sch?nlein purpura nephritis who were clinically diagnosed from the First Affiliated Hospital of Zhengzhou University from July 2014 to July 2016 were enrolled in this study.According to the different levels of proteinuria,HSPN group was divided into two subgroups: a large number of proteinuria group and non-large proteinuria group.According to the differentpathological grades,HSPN group was divided into experimental group Grade ? and?,experimental group Grade III and above.10 cases(n = 10)of children with renal tumor resection were selected as normal control group.15 patients(n = 15)who were diagnosed MCN by renal biopsy were selected as MCN group.Collection the kidney pathology report,serum creatinine,blood urea nitrogen,24-hour urine protein quantitative and other clinical data of the patients.Immunohistochemical method was used to detect the expression of Sirt1 and NF-?B p65 in the kidney tissues.The mean optical density(AOD)was measured by IPP software.SPSS21.0 software was used for statistical analysis.Results1.Sirt1 was expressed in the glomerular of renal tissue in the control group.There was no significant difference between control group and MCN group(P> 0.05).NF-?B p65 expressed little in the control group glomerular,there was no significant difference between control group and MCN group(P> 0.05).2.Sirt1 AOD in the HSPN group was lower than that in the control group and MCN group,the difference was statistically significant(Z =-3.82,-4.40,P <0.05).3.NF-?B p65 AOD in the HSPN group was higher than that in control group and MCN group,the difference was statistically significant(Z = 4.91,-5.63,P <0.05).4.Sirt1 AOD was negatively correlated with NF-?B p65 AOD in the HSPN group(r =-0.885,P <0.05).5.Subgroup analysis of HSPN: Sirt1 AOD in a large number of proteinuria group was lower than that in non-large proteinuria group,the difference was statistically significant(t=-3.78,P < 0.05),NF-?B p65 AOD in a large number of proteinuria group was higher than that in non-large proteinuria group,the difference was statistically significant(t=3.95,P<0.05);Sirt1AOD in group Grade?and ? was higher than that in group Grade III and above,the difference was statistically significant(t=8.88,P<0.05),NF-?B p65 AOD in group Grade?and ? was lower than that in group Grade III and above,the difference was statistically significant(t=-7.88,P<0.05).6.Sirt1 AOD was negatively correlated with renal pathological grade(r =-0.759,P <0.05),serum IgA levels(r =-0.442,P <0.05)and urinary protein levels(r =-0.650 P <0.05).There was no correlation with creatinine,blood urea nitrogen and renal IgA deposition(P> 0.05).Conclusion1.The Sirt1/NF-?Bp65 pathway that inhibited inflammatory reaction may play a protective role in the development of Henoch-Sch?nlein purpura nephritis.2.The level of Sirt1 which expressed in the glomerular can reflect the degree of glomerular damage in patients with Henoch-Sch?nlein purpura nephritis.