| Background: Recurrent spontaneous abortion(RSA)is a common infertility disease in women of childbearing age.The exploration of its causes and treatment has been a hot topic in clinical research.The common causes such as chromosomal factors,endocrine factors,infection factors,genital anatomy factors are knew by scholars.The most active mechanisms of immune-type RSA are related to antiphosphospholipid syndrome(APS),which can activate platelets and mediate the hypercoagulable state of blood.The final outcome is leading to multiple thrombosis of the placenta and resulting in fetal oxygen supply and undernutrition death.The anti-nuclear antibody(ANA)and extractable nucler antigen(ENA)research is relatively small.The mechanism is not clear at present.The purpose that existence of such autoantibody group mainly is immune activation,So that the placenta and decidual blood vessels are damaged,leading to miscarriage.Objective: To understand the relationship between anticar-diolipin antibodies(ACA),ANA and ENA with RSA,To analyze the diagnostic value of ACA combined with ANA and ENA in recurrent spontaneous abortion.Methods: 80 patients of recurrent spontaneous abortion were selected as the observation group from the The Second Affiliated Hospital of Dalian Medical University from January 2017 to January 2017.80 healthy women were selected as the control group.Enzyme linked immunosorbent assay was used to detect the results of ACA in two groups.The ANA results of two groups of subjects were detected by indirect immunofluorescence assay.The ENA spectra of the two groups were detected by enzyme-linked immunospot assay.The data of each group were analyzed by SPSS19.0 statistical software package Statistical analysis,P <0.05 was considered statistically significant.Results: 1.In the observation group,10 patients were positive for ACA and the positive rate was 12.5%;17 patients were positive for ANA and the positive rate was 21.3%;8 patients were positive for ENA and the positive rate was 10%.There were 4 cases of SSA positive,3 cases of SSB positive,1 case of Ro52 positive,2 cases of dsDNA positive,1 case of positive protein,rRNP positive in 1 case.In control group,2 cases were positive for ACA and the positive rate was 2.5%;3 cases were positive for ANA and the positive rate was 3.8%;positive control group is 0 cases for ENA.The positive rate of ACA,ANA,ENA in observation group and control group was significantly higher than whic in control group,P <0.05.The positive rate of ANA was statistically significant.The data of anti-SSA,SSB,dsDNA,Ro52,rRNP,histone,nRNP / Sm,Sm,Scl-70 and Jo-1 doesn't have statistically significant(P> 0.05).2.Statistical analysis of ANA / ACA,ENA / ACA,ANA / ENA.ANA + / ACA +,ANA-/ ACA-,ANA + / ACA-were statistically significant in observation group and control group(P <0.05).There was no significant difference in ANA-/ ACA + between the observation group and the control group(P> 0.05).The difference which ENA-/ACA-in the observation group and the control group was statistically significant(P<0.05),There was no significant difference in ENA + / ACA +,ENA-/ ACA +,ENA + / ACA-between the observation group and the control group(P> 0.05).ANA + / ENA +,ANA-/ ENA-,ANA + / ENA-were statistically significant(P <0.05),and ANA-/ ENA + was no statistically significant difference between in observation group and control group Significance(P> 0.05).3.The positive rate of ACA,ANAand ENA was 9.1%,25%and 6.8% with abortion 2 times.The positive rate of ACA,ANAand ENA was 16.7%,16.7% and 13.9% exceeding abortion 2 times.There was no significant difference in the positive rate between the two groups(P> 0.05).Conclusion:1.The positive rate of ACA,ANA and ENA in the observation group was significantly higher than whic in the control group.The difference between them was statistically meaningful.Forasmuch,ACA,ANA and ENA may be related to recurrent spontaneous abortion and could be used as one of the immunological indexes of RSA etiology.2.There were no significant differences in SSA,SSB,dsDNA,Ro52,rRNP,histone,nRNP / Sm,Sm,Scl-70 and Jo-1 in the ENA spectrum.3.This study suggests that ANA can increase the detection range of RSA diagnosis,ENA diagnosis failed to increase the range of RSA,for the early diagnosis of RSA,the detection sensitivity of ANA is higher than ENA,the abortion can occur early in the disease prediction.4.There was no significant difference in the positive rate of ACA,ANA and ENA between the patients with different abortions,suggesting that there was no significant correlation between ACA,ANA and ENA. |
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