Quality Evaluation And Yellow Pigment Preparation Of Gar Denia Jasminoides Ellis

Objective:The dried and ripened fruits of Gardenia jasminoides Ellis were s elected as the object of study.We select the content of which is rich in crocin,iridoid terpenes and other active ingredients as an evaluation index to study t he quality evaluation of Gardenia jasminoides Ellis.In this way,the suitable habitat and growth period of Gardenia jasminoides Ellis are optimized.At the same time,the process of extraction and purification of gardenia yellow pigm ent were studied.And finally establish a stable,controllable gardenia yellow p igment quality evaluation parameters to provide scientific reference for the in dustrial production of gardenia yellow pigment.Methods:A total of 16 batches of Gardenia jasminoides Ellis were studied b y ultra performance liquid chromatography?UPLC?and pattern recognitio n system.The UPLC fingerprints of gardenia from different origins were esta blished.And evaluated by similarity evaluation,principal component analysis?PCA?and partial least squares discriminant analysis?PLS-DA?to cho ose the appropriate origin gardenia.The contents of geniposide and crocin-I w ere determined by UPLC method.According to the different maturity stages o f gardenia fruit.A total of 15 batches of gardenia were collected from the fruit trees of 1³,which was green and yellow,yellow fruit,yellow and red period,to measure the content and find the change of the active ingredients in garden ia,to determine the optimal period of extraction and purification of gardenia y ellow pigment.According to the literature and pharmacopoeia,the purity and transfer rate of crocin-I and crocin-II;yield,color price and OD value of gard enia yellow pigment were selected.The extraction method,the hot reflux extr action method and the ultrasonic extraction method were investigated by the c omprehensive scoring method.A suitable extraction method was selected.Usi ng L9?3⁴?orthogonal design test.The effects of ethanol concentration,extr action time,ethanol dosage and extraction times were investigated to determi ne the optimal extraction process and carried out the process validation.As th e content of crocin-I in the purification process is high,the content of crocin-I I is very low,sometimes lower than the detection limit,so the choice of crocin-I as an indicator.AB-8,D101,HP-20,X-5,HPD-100 were selected to decide the appropriate model macroporous resin by the comparison of the adsorption amount and resolution of crocin-I.Through the determination of the purity an d transversion rate of crocin-I,the adsorption conditions and analytical conditi ons were investigated,and the purification process was finally determined and the process was verified.The method of thin layer identification of gardenia yellow pigment was determined by the study of the yellow pigment,identifica tion,and content determination of gardenia yellow pigment.The water,incan descent residue,color and OD of gardenia yellow pigment were determined.Crocin-I content determination,the formation of gardenia yellow pigment qua lity standards draft.Results:Through the study of UPLC fingerprints of Gardenia jasminoides E llis from different habitats,the similarity of 16 batches of Gardenia jasminoid es was evaluated by using the geniposide?NO.6?as the reference peak,and the similarity was above 0.9.The similarity of gardenia in Zhejiang was abov e 0.99,and the other groups were below 0.99,which indicated that the differe nce between Zhejiang province was small and the quality was stable.The prin cipal component analysis was carried out by PCA and PLS-DA.The results w ere consistent with similarity evaluation.So choose the origin of Zhejiang gar den for the appropriate origin;The results showed that the content of erythroc yanic acid was 1.21%and that of geniposide was 9.85%,so the selection of g ardenia was the appropriate period;Determine the appropriate extraction meth od for ultrasonic extraction,the optimum process was determined by orthogon al test:the extraction was carried out with 14 times ethanol in 70%ethanol tw ice a time for 1 hour;Identified a suitable macroporous resin model D101 ma croporous resin and purification process,the sample flow rate was 1 BV·h^-1,t he sample volume was 3 BV,and the sample was adsorbed for 2 h,and the wa ter-soluble impurities were removed by distilled water.The geniposide was re moved by elution with 10%ethanol in a volume of 3.5 BV,followed by elutio n of 2.5 BV at a flow rate of 1 BV·h^-1 with 70%ethanol.Three batches of pilo t-scale experiments were carried out,and the results showed that the process was stable and feasible.The eluate was collected,concentrated and lyophilize d.Determination of gardenia yellow pigment thin layer identification method,which stipulated that the water shall not exceed 9%,incineration residue shall not exceed 1%,the color price shall not be less than 400,OD value shall not exceed 0.4,stipulated gardenia yellow pigment-I content of not less than 70%.Conclusion:The results of the study on the quality of Gardenia jasminoides Ellis were the scientific basis for the quality evaluation of Gardenia jasminoid es Ellis.The results of the extraction process,purification process and quality standard of gardenia yellow were the result of the future production of gardeni a yellow pigment.Reference data.