The Study Of The Anti-body Of Connective Tissue Growth Factor On The Inhibition Of Capsular Contracture

Capsular contracture remains the major complication of aesthetic and reconstructive plastic breast surgery.To solve this serious complication,some preliminary studys on the use of related drugs were performed.We took the use of a special way that a modified catheter to be inserted into the lacuna around every gel implant.Through this special pipeline,anti-body of Connective Tissue Growth Factor was delivered and continuously acted on capsular contracture.We try to use a new method to solve capsular contracture,and provide a potential strategy for clinical treatment.Objective:To study anti-body of Connective Tissue Growth Factor on the inhibition of capsular contracture.Methods:100 SD rats were individed into 5 groups,each group with a total of 20.Every rat was implanted with one silicone implant beneath the panniculus carnosus muscle.The sample size of each group is 20,and a total of 50 pairs of experimental and control groups.The anti-body of Connective Tissue Growth Factor was injected into the experimental groups and the control groups were injected with normal saline.At the same time,a modified catheter was inserted into the lacuna around every gel implant.The pipeline had many lateral holes,and the distance of it was blind.lml anti-body of Connective Tissue Growth Factor(2ug/0.2ml)was used through the pot and pipeline as the experimental groups during operation and at 1?2?3?6 months;1ml normal saline was infused as the control groups at the same time.After 1?2?3?4 and 8 months,the SD rats were killed under anesthesia,and we performed the biological and physical measurements related to capsular contracture.Histology examinations of capsular contracture were also performed.Results:1.Capsular firmness:(1).Baker scales:Baker scales of the experimental groups were lower than that of the normal groups from 2 to 8 months(p<0.01).Baker scales increased gradually and reached the peak in the experimental groups and the normal groups in 4 months(p<0.05).(2).Implant compression:Implant compression lowered gradually in the experimental groups or the normal groups in 3 months(p<0.01).There were no evident change after 4 months(p>0.05).Implant compression of the experimental groups were higher than that of the normal groups from 2 to 8 months(p<0.01).(3).Capsular thickness:Capsular thickness thicken gradually in the experimental groups or the normal groups in 3 months(p<0.01).There were no evident change after 4 months(p>0.05).Capsular thickness of the experimental groups were thinner than that of the normal groups from 2 to 8 months(p<0.01).(4).The more lower Baker scales were,the more higher implant compression,and the thiner capsular thickness(p<0.01).2.Capsular collagen content and type:(1).Capsular collagen content:Capsular collagen content increased gradually in the experimental groups and the control groups in 3 months(p<0.01).Capsular collagen content was no significant difference between the two groups in 3 months(p>0.05).Capsular collagen content of the experimental groups were evidently lower than that of the normal groups from 4 to 8 months(p<0.01).(2).Capsular collagen ?/? type:Capsular collagen ?/? type increased gradually in the experimental groups and the normal groups in 3 months(p<0.01),and there were no evident change after 4 months(p>0.05).Capsular collagen ?/? type of the experimental groups was higher than that of the normal groups at the same time points(p<0.01).3.Capsular histology examinations:Capsule was divided into two layers,the outer was loose and the inner layer was dense.As time goes by,the component of cellular was less,and the component of collagen was more.Capsular was thicker gradually in the experimental groups or the control groups in 3 months,and there were no evident change after 4 months.Histology revealed a thinner and more flexed capsular around the silicone implants of the experimental groups as compared with that of the normal groups.There was an increase in the proportion of type ? collagen in the capsulars around the silicone implants of the experimental groups verus that of the normal groups.Conclusions:We took the use of a special way that a modified catheter to be inserted into the lacuna around every gel implant.Through this special pipeline,anti-body of Connective Tissue Growth Factor was delivered and continuously acted on capsular contracture.We found that the anti-body of CTGF had the effect of inhibiting capsular contracture.