Effects Of Erythropoietin In Airway Inflammation Of Hairstyle Asthmatic Mouse Model

Objective:To study the effects and the possible mechanism of erythropoietin(EPO)in airway inflammation of hairstyle asthmatic mouse model,and then to provide experimental and theory basis for clinical application of EPO preventing and controlling asthma.Methods:Select 50 BalB/c mice as the object of this study,which were aged 6 to 8 weeks,and randomly divided into 5 groups:A group(normal control group),B group(asthma model group),C group(dexa met has one group),D group(EPO low concentration group)and E group(EPO high concentration group).Each group has nine mice.Besides using normal saline instead of group A,the rest of the groups of mice were injected 20?g ovalbumin(ovalbumin,OVA)+2 mg of aluminum hydroxide 02 ml on day 1,8,15 day after the start of the experiment intraperitoneal,total sensitization 3 times.And on the 23rd day with 3%OVA solution(saline preparation)inhalation inhalation induced asthma mice,inhaled 30 minutes a day for total 7 days.C group?D group and E group were injected with dexamethasone(1 mg/Kg),low concentration EPO(500 IU/Kg)and high concentration EPO(2000 IU/Kg)30 min before inhalation.Mice were excited to show such as scratching the nose,restlessness,shortness of breath,cyanosis and other typical symptoms of asthma,indicating that the successful production of asthma model.The time and method of intraperitoneal injection and aerosol inhalation in the normal control group were consistent with those of other groups except for the use of saline instead of drugs in addition.The apoptosis rate of eosinophilia(EOS)in alveolar lavage fluid was detected by TUNEL apoptosis in situ,and the levels of IgE and TGF-?1 in the serum were measured by enzyme-linked immunosorbent assay(ELISA).The lung tissue of mice after dealing with the section,HE stain,the corresponding pathology morphological changes were observed under optical microscope.Results:Compared with group A,the apoptosis rate of EOS in group B was decreased,and the levels of C,D and E were significantly increased;the levels of serum IgE and serum TGF-?1 in group B were significantly lower,and the differences were statistically significant(P<0.05).Compared with group B,the apoptosis rate of EOS in group C,D and E was significantly higher,the levels of serum IgE and serum TGF-?1 were significantly decreased(P<0.05).Compared with group C:1)the apoptosis rate of EOS of group D was increased,IgE and TGF-?1 were decreased(P>0.05);(2)the apoptosis rate of EOS in E group was significantly higher,IgE and TGF-?1 were significantly decreased(P<0.05).Compared with group D,the EOS apoptosis rate in group E was significantly higher,and the levels of IgE and TGF-?1 were significantly decreased(P<0.05).(2)The lung tissue of mice was stained by HE after light microscopy:A group was abnormal,alveolar size was uniform,alveolar septum thin,alveolar capillary rich,bronchial epithelium no significant damage,no inflammatory response.B group was significantly changed,there are lint lavage,loss,pulmonary vascular proliferation,pulmonary interstitial edema,and visible more inflammatory cell infiltration,and most of the focus on the bronchus and its blood vessels around,eventually leading to the stenosis of the lumen.The results of D and E showed that the degree of inflammatory response and luminal stenosis were lower than those of group B,and the degree of pathological damage was decreased in a dose-dependent manner with the increase of EPO concentration.Compared with group D and E,Bronchial lumen and smooth muscle thickness change has no obvious difference,but the group C significantly reduce inflammation degree.Conclusion:EPO can promote the apoptosis of EOS in bronchoalveolar lavage fluid of asthmatic mice,decrease the level of serum IgE and serum TGF-?1 and reduce the pathological changes of lung tissue in asthmatic mice,and had an inhibitory effect on airway inflammation in asthmatic mice.