The Research Of TCM Consolidation Treatment Theory And It's Effects To New Neurons In Kidney Deficiency AD Rats

ObjectiveOrgin qi is the most basic material for life sustaining activities,also the driving force of human life activities.The vitality of congenital essence from parents,hidden in the kidney and the Mingmen.Epidemiological studies also show that AD is the most common disease in patients with kidney deficiency syndrome.Traditional Chinese medicine believes that the kidney empty is the root of the AD,and the "treatment from the kidney" as the main treatment.This paper starts with reinforcing Orgin Qi,kidney deficiency type senile dementia as a breakthrough point.To explore the relationship between Orgin qi and kidney deficiency,and vitality consolidation treatment of kidney deficiency type senile dementia effects.kidney deficiency type senile dementia rats as the animal model,To observe the vitality consolidation compound include Changsheng Guben compound,Guben Jiannao mixture and Peiyuan Guben capsule effect the concentration of A beta 1-40 and A beta 1-42 in hippocampus of rats with kidney deficiency dementia and Study on the increment and maturation of newborn neurons.Objective to provide theoretical basis and experimental data for the clinical treatment of senile dementia.Method:1.Theoretical discussion: Collection of literature on the relationship between Orgin qi and kidney deficiency dementia and The literature on the increment and maturation of newborn neurons.To explore the theoretical basis of the reinforcing orign qi to treate kidney deficiency senile dementia.2.Experimental study: Kidney deficiency senile dementia rats as an animal model,the hippocampus of newborn neurons increase and the mature as the object of investigation,Study on reinforcing the vitality of traditional Chinese medicine compound Changsheng Guben compound,Guben Jiannao mixture and Peiyuan Guben capsule impacts on Kidney deficiency senile dementia rats.Evaluation of kidney deficiency dementia model with data of body weight,corticosterone,testosterone and water maze,The ability of learning and memory of rats was detected by Morris water maze navigation experiment and space exploration experiment,Detection of A beta 1-40 and A beta 1-42 protein in hippocampus of rats by ELISA,Brdu immunohistochemical analysis of the three party in the kidney deficiency AD rat model of newborn neurons.Result:1.Changes in weight and corticosterone and testosterone levels: Compared with the control group,the weight of model group rats with hydrocortisone injection did not even reduce weight gain;Compared with the blank group,the serum corticosterone level in the model group was significantly lower than that in the blank group(*p <0.05).Compared with the blank group,the serum testosterone level in the model group was significantly lower than that in the blank group(**p <0.01).Combined with body weight and general condition,the kidney deficiency AD rat model was successful.2.Morris water maze test results:Compared with the model group,the rats in each group were shortened(p<0.01,p<0.05),the total swimming distancewas reduced(p<0.01,p<0.05),Compared with donepezil group,have no difference in Changsheng Guben compound and Guben Jiannao mixture on the platform latency and swimming distance(p>0.05),Peiyuan Guben capsule group on the platform with no obvious change in latency(p>0.05),increasing the total swimming distance(C<0.05);Compared with the model group,the total number of crossing platform and target quadrant time of the other groups were significantly increased(p<0.01,p<0.05),the first time was significantly shorter(p<0.01,p<0.05).Compared with donepezil group,Changsheng Guben compound rats in fixed quadrant time and the first time to reach the platform were not significantly different(p>0.05),after the number of platform area is relatively reduced(p<0.05);The Guben Jiannao mixture group rats through the total time and the number of platform target area have no significant difference(p>0.05),the first time arrived at the platform for a relatively long time(p<0.01);Peiyuan Guben capsule group no difference across the platform(p<0.05),the target area total time(p<0.05),the first time arrived at the platform for a relatively long time(p<0.05).3.Content of A beta 1-40 and A beta 1-42 protein in hippocampus of rats:Compared with the blank group,model group,donepezil group,Changsheng Guben compound group rat hippocampal tissue A beta 1-40 decreased obviously(p<0.01),reduced the Guben Jiannao mixture group and Peiyuan Guben capsule group rats hippocampus A beta 1-40 content(p<0.05);Compared with donepezil group,Changsheng Guben compound group A beta1-40 content had no obvious abnormalities(p> 0.01),increased the Guben Jiannao mixture group and Peiyuan Guben capsule group A beta 1-40 content(p<0.05);Compared with the blank group,model group,donepezil group,Changsheng Guben compound group,the Guben Jiannao mixture group rats A beta 1-42 decreased obviously(p<0.01),reduced Peiyuan Guben capsule group rats hippocampus A beta 1-42 content(p<0.05);Compared withdonepezil group,Changsheng Guben compound group,the Guben Jiannao mixture group A beta 1-42 content had no obvious abnormalities(p> 0.01),elevated Peiyuan Guben capsule group A beta 1-42 content(p<0.05).4.The value of newborn neurons and the number of mature cells: Compared with the model group,blank group,donepezil group,Changsheng Guben compound group and the Guben Jiannao mixture group increased the number of new neurons in mature cells(p<0.05),and Peiyuan Guben capsule group of newborn neurons in mature cells had no significant difference(p>0.05).Compared with donepezil group,Changsheng Guben compound group,the Guben Jiannao mixture group and Peiyuan Guben capsule group had no difference(p >0.05).Conclusion:1.After the modeling,model rats weight increase is not obvious,and even weight loss,low degree of active,inactive,and soft,thriller,hair blown off and obvious.With the continuation of the gradual emergence of drugs,poor activity,loose stools,cold,hair,hair gloss and other symptoms;and memory decline,the results showed that the model was successful.2.The Changsheng Guben compound,the Guben Jiannao mixture and Peiyuan Guben capsule can improve the rat model to a certain extent,the learning and memory function,and no significant differences.3.The Changsheng Guben compound,the Guben Jiannao mixture and Peiyuan Guben capsule can decrease the hippocampus A beta 1-40 and A beta1-42 protein,the Changsheng Guben compound,the Guben Jiannao mixture is the best solid role Peiyuan capsule.4.The Changsheng Guben compound,the Guben Jiannao mixture and Peiyuan Guben capsule could promote the proliferation and maturation of neurons,and no obvious difference,can effectively improve the symptoms of dementia.