| Purpose:Explore the effect and mechanism of Pi-Xu-Zhang-Man Mixture on sp leen-deficiency constipation miceMethod:According to the randomized digital meter,72 kun ming mice were di-vided into 6 groups.They are blank group, model group, positive control group, Pi-Xu-Zhang-Man Mixture low, medium and high dose group.Except the blank group, each group of mice by adopting the comprehensive method of "Bitter and cold medicine cause diarrhea+Hunger disorder+Water shortage cause faeces dry"comprehensive copy spleen-deficiency constipation mice model.After the success of the model, the treatment groups were given corresponding drug lav-age,the blank group and model group were given with the same amount of d-istilled water to fill the stomach,7 days of treatmentRecord the change of th-e general situation of mice during the experiment.After the last delivery,take th-e eye blood and cut open the abdomen for stomach, duodenum, small intestin-e, spleen, observe the rate of gastric emptying and small intestinal propulsion r-ates and the spleen index changes.Using enzyme-linked immunosorbent method to detect the mice serum CCK and duodenum MLCK, CAM content changes.Results:1.General conditions:Blank group of normal mice activities, defecate is normal, responsive, good appetite, glossy back feather,weight increased signific-antly and no deaths.Establishing the type 2-7 days gradually appear loose faeces,anus week filthy, malaise tiredly, cluster, food intake less, activity less;even suede f lew up, sparse back feather lose luster, bent back,and weight gain slo-wly.Esta blishing 8 days began to appear gradually faeces fewer, particles less, t-he qual ity more harden, conform to the constipation symptoms of spleen-defi-ciency.A fter the treatment, the type diet increase gradually, other conditions gra-dually i mprove.Weight:Before building the type weight comparison, there was no obvious statistical significance difference (P> 0.05), comparable.After building, the buil-ding module weight in mice are much smaller than normal group (P< 0.01). Prompt the comprehensive method of "Bitter and cold medicine cause diarrhea +Hunger disorder+Water shortage cause faeces dry" copy spleen-deficiency con-stipation mice modell can slow weight gain in mice.After treatment, the traditi-onal Chinese medicine treatment group, positive control group and model grou-p mice weight are less than normal group (P< 0.05).And each treatment gro-up mice weight is greater than the model group (P< 0.05).2.Rate of gastric emptying and intestinal propulsion:Compared with the blank group, model rate of gastric emptying and small intestinal propulsion rat-es of mice were significantly lower (P< 0.01).Prompt the comprehensive meth-od of "Bitter and cold medicine cause diarrhea+Hunger disorder+Water shortag-e cause faeces dry" can reduce rate of gastric emptying and small intestinal propulsion rates in mice.Compared with model group, the treatment rate of gast-ric emptying and small intestinal propulsion rates of mice increased (P< 0.0 l).Compared with positive control group, bloated with mixture of pixu (spleen deficient) dose rate of gastric emptying and intestinal propulsion rate of mice s-ignificantly increased (P< 0.05)3.The spleen index of mice:Compared with the blank group, model group mice spleen index significantly decreased (P< 0.01).Prompt the comprehensive method of "Bitter and cold medicine cause diarrhea+Hunger disorder+Water sh-ortage cause faeces dry" can reduce spleen index in mice.Compared with mod-el group, the Chinese medicine treatment group mice spleen index significantly increased (P< 0.01 or P< 0.05), no difference between the positive control-group mice spleen index (P> 0.05).4.The mice serum CCK content and duodenum MLCK content>. duodenu-m CAM content(1) Serum CCK content:Compared with the blank group, model group mice serum CCK content increased significantly (P< 0.01).Prompt spleen-defi -ciency constipation mice serum CCK content has a rising trend.Compared with model group, the western medicine control group and traditional Chinese medi-cine (TCM) mice serum CCK content in each treatment group were lower, P< 0.01 or P< 0.05;Compared with positive control group, the Chinese medicin-e treatment group mice serum CCK content has no obvious difference (P> 0. 05).(2) Duodenum MLCK content:Compared with the blank group, model gr-oup mice duodenum MLCK content significantly decreased (P< 0.01).Prompt spleen-deficiency constipation mice duodenum MLCK content has a downward trend.Compared with model group, the treatment group mice duodenum MLC K content increased (P<0.01 or P< 0.05).Compared with positive control gro-up, the medium dose of Chinese medicine treatment group mice duodenum ML-CK content increased significantly (P< 0.05).(3) Duodenum CAM content:Compared with the blank group, model grou-p mice duodenum CAM content levels increased significantly (P< 0.0^.Prom-pt spleen-deficiency constipation mice duodenum CAM content has a rising tr-end.Compared with model group, the medium and high dose of Pi-Xu-Zhang-M-an Mixture treatment and western medicine group mice duodenum CAM conte-nt significantly decreased (P<0.0l or P< 0.05).Compared with positive contr-ol group, the medium dose of Chinese traditional medicine group duodenum C-AM content significantly decreased (P< 0.05).Conclusion:Pi-Xu-Zhang-Man Mixture can improve the clinical manifestations o-f spleen-deficiency mice with constipation, slow growth in weight, eating less,f-eather lose luster and weak tiredly, treating constipation model mice gastrointe stinal disorders, improving gastric emptying and small intestine propulsion funct-ion and the immune function in spleen-deficiency constipation mice.Its mecha-nism may be related to lower spleen-deficiency constipation mice serum CCK content and the content of the duodenum CAM, increase the content of the d uodenum MLCK. |
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