The Investigation Of Anti-Inflammatory And The Mechanism Of Trillium Tschonoskii Maxim To Collagen-Induced Arthritis Rats

Objective To evaluate the therapeutic effect of trillium tschonoskii maxim(TTM)on collagen-induced arthritis and its effect on the expressions of inflammatory cytokines,vascular endothelial growth factor,and cyclooxygenase in rats,and to explore the mechanism involved,laying the foundation for the development of new drugs for RA treatment and the exploitation of genuine Chinese herbal medicine.Methods 70 male SD rats were included in this study,of which 60 rats were used in the establishment of type II collagen-induced arthritis rat model.To observe TTM for prevention of collagen induced arthritis,20 rats divided into preventive medication group and model control group,The first time in the immune were given doses of TTM or saline at the same time,Drug delivery to 1 week executed after the second immunization。To observe TTM to the CIA’s therapeutic effect,divided into 40 rats including 3 treatment groups and model group,the second immune evaluation made after the success of the model were given different doses of TTM or saline,for daily for 4 weeks after death.Arthritis index and pathological changes of rats were observed,immunohistochemical assay was employed to detect the expressions of VEGF,TGF-1β,COX-1,and COX-2,and expressions of serum IL-6,IL-1,IL-17,and TNF-α in CIA rats before and after treatment were detected by ELISA。Results 1.Joint swelling index:High dose group compared with model group si gnificantly reduced(P<0.01),Low dose is reduced,but the difference was not si gnificant,prevention group compared with model group significantly reduced(P<0.01).2.The following changes were noted in the model group by HE and ot her special staining:(1)Hyperplasia of synovial cells.Increased layers and disor derly arrangement of synovial cells were found and cell polarity disappeared.(2)Infiltration of inflammatory cells.Lymphocyte-dominated inflammatory cell i nfiltration scattered in synovium,with a small amount of plasma cells;aggrega ted or scattered lymphocytic infiltration was seen around blood vessels and vas cular wall;scattered lymphocytic infiltration was found in interstitial matrix.(3) Hyperplasia of fibrous tissue,mainly manifested as the hyperplasia of collagen fibers.Fibrous tissue was arrayed densely with a disorderly arrangement.In s ynovial cell hyperplasia,inflammation cell count,blood capillary hyperplasia,fi brosis and amyloidosis pathological changes of five comprehensive score and c omparative analysis,visible pathological and histological changes in treatment g roup compared with model group have different degrees of improvement,espec ially in high dose group was most pronounced(P<0.05),Histologic change prev ention group was obviously lighter than model group(P<0.05).3.Synovial tissu e immunohistochemical examination:expression of VEGF,TGF-1β,COX-1,COX-2in the normal group were negative,Model group Dyed darker,Dyeing range an d number of positive cells.The expression of the above indices in synovial tis sue staining intensity and positive index score after the comparison,The index was weakly positive prevention group,coloring,was lower than those of mod el group(P<0.05).Treatment group expression is abate,in high dose group of t he most significant,positive cells was significantly reduced,parts of negative e xpression,staining intensity is small(P<0.05).4.The expressions of serum IL-6,IL-1,IL-17 and TNF-α in the model group were higher than the normal grou p(P<0.05).The levels of all these cytokines were reduced after treatment,and particularly that of the medium-and high-dose treatment groups and the preve ntion group were significantly decreased(P<0.05).Conclusion In advance to TTM can significantly reduce type II collagen induced arthritis rats,To give TTM to the CIA model rats can obtain certain therapeutic effect.TTM improves the pathological changes of synovial tissue in CIA rats,and inhibits the proliferation synovial cells to reduce the angiogenesis in synovium,reduce the symptoms of arthritis,and control the fibrosis process by regulating the expressions of inflammatory cytokines,VEGF,and COX,so as to exhibit the preventive and therapeutic effects in CIA rats.