| Objective:In this research,studide on electro-acupuncture treatment of rheumatoid arthritis theory,clinical efficacy was observed and observed the levels of IL-1,IL-4,IL-6.IL-10,TNF-αin the synovial fluid of rats,at the same time,compared with the regular acupuncture.This research discussed the mechanism of acupuncture treating RA.Methods:Theoretical Study:Systematically expounded on the understanding of rheumatoid arthritis as well as discussion of pathogenesis.This research focus on the progress of the treatment of rheumatoid in traditional Chinese medicine especially acupuncture,and the progress of rheumatoid arthritis animal models.Clinical research:The diagnostic standards of RA and evalution standards state from American College of Rhenmatoligy(ACR) were used in this study.This study of 27 cases of electroacupuncture treatment effect observed in patients with RA and with the regular needle acupuncture group 28 cases of RA patients with control.Points of overall selection,along with the actual points of pain on the body,were used.Acupuncture treatment was applied every other day for a total of 30 days with a 2 days intermission after the 10th day.The two groups were observed before and after treatment according to the evaluation standards of illness state from American College of Rheumatology(ACR),and the same time,testing laboratory indicators.Animal experiments:60 Wistar male rats with body weights from 230g to 270g were divided into four groups as follow:normal control(20rats),Collagen-induced arthritis(CIA)withouttreatment(20rats),CIA with eletroacupuncture treatment(10rats),CIA with regular acupuncture treatment(10rats).Acupuncture treatment was performed every day 21days after modeled,points of Yanglinquan,Huantiao,Bafeng,Shenshu of both sides were choiced.After pulling the needles into the two sides of Yanglingquan connected with electronic acupuncture equipment.The treatmen lasted 20 minutes each time for 20 times with 2 days intermission after the 10th treatment.The four groups were observed before and after model and treatment the general situation in rats,foot circumference,arthritis index(AI),levels of cytokine including IL-1,IL-4,IL-6,IL-10,TNF-αin synovial fluid.Results:Clinical observation and experimental study has been made more consistent with observations.Clinical research:1.Electroacupuncture improved ACR-20 of 71.43%patients and improved ACR-50 of 21.43%of patients.This was significantly different from the effects that were achieved byregular acupuncture,which improved ACR-20 of 48.15%patients and ACR-50 of 14.81%patients(P＜0.05).2.The two groups were significantly decreased the levels of IL-1,IL-6,TNF-αand improved the levels of IL-4,IL-10 in RA patiens's synovial fluid(P＜0.05),while electroacupuncture was more effective than regular acupuncture in improving the level of IL-4(P＜0.05),but without siginificant difference in this two groups(P＞0.05).Animal experiments:1.The CIA rats' foot circumference and arthritis index was significantly increased(P＜0.05).These indicators significantly decreased after electro-acupuncture treatment(P＜0.05).2.The levels of IL-1,IL-6,TNF-αwere significantly increased in CIA rats' synovial fluid,and the levels of IL-4,IL-10 were significantly decreased(P＜0.05).After electro-acupuncture the levels of IL-1,IL-6,TNF-αwere significantly decreased,and the levels of IL-4,IL-10 were significantly increased(P＜0.05).Conclusions:1.Electroacupuncture improved ACR-20 of 71.43%patients and improved ACR-50 of 21.43%of patients.2.Electro-acupuncture can decrease the levels of IL-1,IL-6,TNF-αin RA patients' synovial fluid,and increase the levels of IL-4,IL-10.This change to repair the joints of patients with positive significance.3.The animal test of the effect of electroacupuncture on cytokines of CIA rats and the clinical observation has been made consistent results.4.Changes in cytokines is one of the inherent mechanism in acupuncture and moxibustion treatment of rheumatoid arthritis. |
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