Method Validation And Kinetic Analysis Of The Silver Release From Excipients In Horse Serum

Purpose Through effective method to show the silver ion dressings slow-release effect and the actual amount of slow-release,and further illustrate the dressings gradually release silver ions,continuous,broad antibacterial,security,environmental protection and no resistance.Silver dressing replacement medicines reduces the transfer of epithelium and granulation tissue new mechanical damage to the stimulation of nerve endings,and reduce their pain during dressing change,increased comfort,decrease the switching frequency,shorten the treatment time and enhance other excellent properties.Silver dressing general medical on burn wounds,the release of the silver level and the serum concentrations of serum absorbed directly affect the treatment effect of silver dressings.Silver dressing sustainable release of the silver ions and obtain good antibacterial effect.Particularly susceptible to burn wound infection,so use of silver on burn wound dressing of silver release quantity is high,can maintain a relatively high concentration of silver in the wound.From the dressing on the skin,after the release of silver ions with protein to form temporary "silver library",silver and protein formationof the complex is colorless.The "silver library" can extend the antibacterial effect.In high hygroscopicity of medical dressings,bacteria and wound exudates are sucked into dressing,a small amount of silver ions released from the dressing can achieve the goal of antibacterial.Method The total silver present in the impregnated dressings is extracted using horse serum,then acid digestion the extracts and serially diluted volumetrically to give a concentration range in the optimum region for analysis by inductively coupled plasma.This method will be validated experimentally and rationally with respect to accuracy,linearity,precision,robustness,specificity and range.The dressing will be analyzed by method validated.Assuming that the levels of silver released do not change over time analysis of variance(ANOVA)testing may be carried out to show that there is not a statistically significant difference between all data sets collected and thus show that the level of silver released is consistent regardless of the site of manufacture.ANOVA testing was carried out at the 95% significance level.The silver release level is equivalent both dressings and can achieve the desired therapeutic effect in the treatment period.Result Successful Validation of test containing silver dressing the release of the level of test method.And through this method to analyzethe batch of 25 different kinds of silver dressing,using ANOVA compares the test results.Each type of product after 8 days of testing,the silver dressing silver release uniform every day,there is no significant difference between levels.Conclusion This study successfully validated the method of containing silver dressing release.Andthrough the effective method to show the silver dressings slow-release effect and the actual amount of slow-release.It has extensive and practical application value.Want to apply this method to evaluate and to make the silver ion dressings and other new type of medical dressing is applied in the domestic market,gradually replace the traditional gauze,bandages,etc.