Study On Preparation And Properties Of Antibacterial Biology Dressing

After being injured, repair and restore of the wound, i.e., the organism function is restored to health, has becoming the premise. How to speed up the course of repairing? Multitude methods have been used or being researched, among which surgical dressing research has been developed into to hot spots.Silk fibroin is an inartificial protein used as textile material for a long time. Recent years, interests have been rising in the use of silk fibroin as starting material for advanced biomedical applications, such as wound dressing and artificial skin, based on its virulence and excellent biocompatibility. However, pure silk fibroin film has a property of frangibility and can be dissolved in water easily, which limited its usage in clinic. In order to extend the field of silk fibroin usage, people have to find a better method.Collagen is one major structural protein in the extra cellular matrix and also one primary component of knot tissues. It accounts for approximately one-third of protein mass in mammal animals and can be found in large quantities in skin, bone, tendon, ligament and blood vessel. With its excellent biocompatibility, biodegradability and weak antigenicity, collagen is widely used in the field of drug delivery and tissue engineering.Silver is one of the most important precious metals. It has been widely used as antibacterial material, medical material, optical material, ornamental material, catalytic material, electrical and electronical materials, etc. Due to their peculiar properties caused from the volume effect, the surface effect, the quantum dimension effect and the macro-quantum tunnel effect, the silver nanoparticles might become to the prospective important materials applied potentially in many field.In this study, a kind of antibacterial dressing was prepared. First, the raw material of the dressing which we meant to build was prepared and extracted from tendon belonging to bull. The aim protein—type I collagen was extracted by using the pepsin-soluble method. To optimize the condition of extraction, the concentration was monitored with Coomassie Brillant Blue dyeing. Data showed that the enzymic hydrolysis was the best when the pepsin to tendons was 1:10. Second, as we know, there are many different kinds of method to refine silk to form doffing sericin. In our study, the silk was in turn preconditioned by papain(0. 2%, the weight of wet silk: the volume of papain solution1:50) and 5g/L Na2CO3. Then silk fibroin was dissolved by the optimum concentration CaCl2 solution(1:50), and the obtained solution was desalted by the dialytic bag. Third, the nanometer-like silver sol was prepared using chemistry reduction in aqueous solution, into which the silk fibroin net was dip and dried to build nanometer silver silk. Diameter of the silver granule was examined by Transmission Electron Microscope(TEM), and the results showed that a nanometer magnitude silver granule had been shaped. Finally, a mixture of typeⅠcollagen solution and silver sol(1:1) was coated on the silk fibroin to prepare the antibacterial biology dressing.A series of experiments were designed and carried on to test the properties of the material, including its physical and chemical characteristics, such as the effect of antibacterial, the tolerance to physiological saline, the gas-permeability and the water permeability. The biological characteristics, such as stimulation of cell proliferation and antibacterial property were also examined. The data show that the antibacterial surgical dressing have have the effect of antibacterial, very steady in physiological saline, agravity rate is 2.5% every 24 hour in 37℃, excellent in gas-permeability and suitable in water permeability, transit dose of water vapor is 2019g/24h·㎡. Besides, the material could stimulate the proliferation of fibroblast in vitro. In conclusion, the antibacterial surgical dressing has the potential of being used in clinic.