Association Study Of FCGR2A Gene Single Nucleotide Polymorphism With Kawasaki Disease In Chinese Han Children

Objective:Based on genome-wide association study,Single nucleotide polymorphism (SNP) rs1801274that markers in FCGR2A gene was found has relationship with KD susceptibility.The relevance was studied in other ethnical groups。But the replication studies to replicate the genetic association between SNP rs1801274in FCGR2A gene and KD susceptibility in Chinese population is inadequate.Our study was designed to investigate the association of the FCGR2A gene SNP rs1801274with Kawasaki disease (KD) susceptibility in Chinese Han childrenMethods:A case-control study was performed. The case group included35children who were diagnosed as KD between October2010and April2012in the Tianjin Children’s Hospital. Twenty-five age-and gender-matched children who came from clinical department waiting for operation were enrolled as the control group. All KD patients were divided into coronary artery lesion(CAL)group (n=13)and no coronary artery lesion (NCAL)group(n=22).All the study subjects are Chinese Han children. Genomic DNA was prepared from peripheral venous blood (2mL) using a genomic DNA isolation reagent kit. Polymerase chain reaction (PCR) and gene sequence analysis were applied to detect the single nucleotide polymorphismof FCGR2A gene rs1801274. The differences about allele and genotype frequencies in different groups were compared.Results:FCGR2A gene SNP rs1801274was detected in all the subjects. There were three genotypes (AA, AG and GG) at this SNP position.For FCGR2A gene SNP rs1801274, there were significant differences in the genotype frequency(χ2=8.53,P=0.01) and allele frequency (χ2=9.39, P=0.003)between the KD and healthy children (P<0.05).A allele was linked an increased risk for KD susceptibility(OR=3.14). Genotype (χ2=6.82,P=0.03) and allele (χ2=7.13, P =0.01) frequencies for FCGR2A gene SNP rs1801274were different in KD children with CAL and NCAL (P<0.05).G allele (OR=4.88) increased risk for CAL in Chinese Han children with KD.A allele frequency is higher than G allele frequency in Chinese Han KD children without CAL.Conclusions:1. Polymorphisms of FCGR2A gene SNPrs1801274may be associationed with KD susceptibility and with CAL.2. A allele of FCGR2A gene SNP rs1801274may be a risk factor of developing KD.3. G allele of FCGR2A gene SNP rs1801274is thought to increase risk of developing coronary artery lesion in children with KD.