Placental Grb10 Expression And Epigenetic Modification Analysis For Evaluation Of Security For Vitrification Blastocyst Offsprings

Objective: to analyze the obstetric outcome and expression of the placenta Grb10, GCN5 and HDAC1 and DNMT1 from women conceived by transferred thawed blastocyst, and to evaluate the security of blastocysts culture and vitrification.Methods: a cross-sectional study was performed in the hospital from January 2012 to may 2014. 50 women conceived by transferring thawing blastocyst and 50 natural pregnancy control women were enrolled in this study. Immunohistochemical staining was performed for detection of the placental expression of Grb10 and GCN5 and HDAC1 and DNMT1; Real-time PCR was performed to detect the Grb10 m RNA expression on placenta; Western blot was performed to analysis the placental Grb10 protein expression.Results: 1. The rate of threatened abortion and occurrence rate of cesarean section increased significantly in women conceived by transferring thawing blastocyst(P < 0.05), but the other major obstetric complications and perinatal risk did not increase(P > 0.05). 2. The area and weight of placenta in women conceived by transferring thawing blastocyst increased significantly(P < 0.05), but the development related molecules Grb10 expression on placenta didn't changed significantly(P > 0.05). 3. There was no significant change of placental expression of GCN5, HDAC1 and DNMT1 in women conceived by transferring thawing blastocyst(P > 0.05).Conclusion: the blastocyst vitrification technology is still a safe and effective assisted reproductive technology.