The Research Of Serum Long Non-coding RNA Uc022bqs.1 In Early Diagnosis Of Human Coronary Heart Disease

AIM:To determine the lncRNA expression profile in serum of coronary heart disease(CHD) patients and to find specific lncRNAs as molecular markers for early diagnosis of CHD.METHODS:1. The difference of lncRNA expression profile between CHD patients and normal individuals was measured by lncRNA microarray.2. QRT-PCR was used to confirm the expression of representative lncRNA in 30 cases of normal control(NC), 30 cases of stable angina pectoris(SAP), 42 cases of unstable angina pectoris(UAP) and 30 cases of acute myocardial infarction(AMI), we then analyzed whether the expression levels of target lncRNA in serum with CHD patients were different.3. The relationship between their levels and pathological factors of patients with CHD was explored. A receiver operating characteristic(ROC) curve was adopted to verify the diagnostic efficiency of the selected lncRNAs.RESULTS:1. LncRNA expression profile showed 224 lncRNAs with expression alteration eight fold or above. The up-regulated and down-regulated candidate lncRNAs were 59 and 165(P<0.005), respectively.2. The expression of serum lncRNA uc022 bqs.1 verified by qRT-PCR was consistent with the microarray analysis.3. Using ROC curves analysis, serum lncRNA uc022 bqs.1 could discriminate the SAP, UAP and AMI from NC and the areas under curve(AUC) was 0.796(95%CI:0.681~0.911), 0.821(95%CI: 0.718~0.924), 0.846(95%CI: 0.795~0.932), respectively.For discriminating the CHD patients from NC, the AUC was 0.895(95%CI:0.824~0.965), and the sensitivity and specificity were 79.5% and 83.3%, respectively.CONCLUSIONS:Serum lncRNA uc022 bqs.1 may serve as a potential marker for the early diagnosis of coronary heart diseases.