Evaluation Of Bone Regeneration By ? Tricalcium Phosphate/Collagen Scaffold Loaded With Human Bone Morphogenetic Proteins 2 Plasmid On Osteogenesis Ability To Influence

Objective With biological cell technology swift and violent development,advancing with The Times more and more scholars and clinical research and development of a variety of different types of osteogenesis bioactive factors,Using genetically engineered tissue therapy to treat bone defect is no longer a dream.In the meantime the development of nanotechnology is also very quickly,More and more clinical workers focus on nano composite scaffold materials in order to become a clinical alternative materials to repair bone defect perfect.Bone tissue engineering scaffold material load has biological activity factor can effectively promote the repair tissue defect,Studies this kind of new scaffold materials to repair bone defect has become a research direction.Can and seed cells and bioactive factor is very perfect combination for the clinical restoration and treatment of bone defect provides a very feasible way.This experiment building contains hBMP2(human ipads morphogenetic proteins2,hBMP2)plasmid DNA beta TCP/collagen(beta tricalciu phosphate/collagen)stent materials,And study the composite scaffold materials for osteogenetic activity ability to former osteogenesis MC3T3-E1 cells in mice.Methods The preparation of nanoscale porous beta TCP/collagen scaffold containing and load BMP2 gene plasmid DNA genetic modification of scaffold materials.Composite scaffold and former osteogenesis MC3T3 E1 cell lines in vitro culture system.Will be divided into the stent group(Z)peace melamine(M),According to the different concentration of plasmid again each group is divided into two groups.Scaffold and cell composite training samples by scanning electron microscope after composite scaffold surface morphology,Osteogenesis induced by 1,3,7,14 d testing different concentrations of BMP2 stent group peace dish cells the activity of alkaline phosphatase(ALP),By realtime fluorescent quantitative PCR detection method of different time points in the distribution of induced Runx2,OCN,ALP,OPN osteogenesis markers such as gene expression.Test results for statistical analysis.Results Plasmid DNA containing hBMP2 gene modified nano beta TCP/collagen composite scaffold material surface is porous sample solution structure;Add BMP2 stent group peace dish can improve the activity of ALP and osteogenesis related marker gene expression;Stent group of cells of mice former osteogenesis MC3T3 E1 osteogenesis ability to promote group obviously superior to the dish.Conclusions 1.To hBMP2 gene modified beta TCP/collagen scaffold material has very good bone induced,Is a kind of good repair instead of scaffold materials of tissue defect.2.Load hBMP2 genetic modification of beta TCP/collagen scaffold material biocompatibility to hBMP2,beta TCP and collagen is obviously better than the three kinds of materials,respectively,to the effect of the alternatives to repair bone defect.