| Purpose:Compare the effects of artificial bone containing BMP and collagen and autogenous iliac bone on repairing large segmental radial defects of Zelanian rabbitsMethod:96-month-old healthy Zelanian rabbits were included in this study. Under general anesthesia,6 rabbits were given osteotomy of bilateral radius (or near the end) by the length of 1.5cm interception. All rabbits, the right side (A group) was implanted with artificial bone, while the left side (group B) was implanted with autologous iliac bone. The remaining 3 rabbits (group C) were treated with bilateral radial osteotomy without bone grafting. Immediate,4 weeks,8 weeks and 12 weeks after the surgery the rabbits were taken X-ray film and score the calculation. After 12 weeks of the operation, the animals were sacrificed and the radius was retrieved. The Micro-CT scanning and HE staining were performed to analyze the osteogenesis of the defect. Application of the above methods to compare the clinical effect of two kinds of methods for the treatment of bone defect in the middle section of the rabbit radius.Result:The difference of radiographic scores in the experimental group and blank group are statistically significant 4 weeks to 12 weeks after operation (P< 0.01). While there was no significant difference between the experimental group and control group (P> 0.05); Histological evaluation showed that callus formation was worsen in the experimental group than in the control group; The Micro-CT showed that there were no significant difference in bone volume, bone trabecular pattern factor between the experimental group and control group. (P> 0.05). The blank group cannot produce bony healing.Conclusion:Artificial bone containing BMP and collagen could repair large segmental bone defect with satisfied results, which is almost comparable with autogenous iliac bone graft. |
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