Embryonic Development Stops The Molecular Immune Mechanism Research

Objective Embryonic development stops lead to fetal death and miscarriage, serious damage to the fetus and the physical and mental health of pregnant women. In recent years, the proportion of embryo occurs in pregnant women stop development has a tendency to increase year by year, which not only seriously affect their health, but also cause heavy psychological burden to the family and economic burden.Of this study was to explore the embryo development stops in patients with endometrial tissue Trophinin on the expression of adhesion molecules, immunosuppressive acidic protein (IAP) in the blood, Treg cells and CD4+Th cells content and related factor levels, confirmed its relevance to embryonic development stops, targeted for the molecular immune mechanism of the disease and treatment to provide new ideas.MethodsThe object of study for between January 2014 and December 2014 in jimo maternity and child care women and 76 cases of obstetric treatment will be divided into three groups. Embryonic development stops groups:from 26 patients with embryo development stops;Normal pregnancy group:25 cases of patients with normal embryonic development; Health unfertilized groups:25 cases with normal reproductie history, menstrual cycle normal women of childbearing age. Using immunohistochemical method to detect Trophinin adhesion molecule expression in human endometrial tissue cells;Using single AGAR diffusion method to determine immunosuppressive acidic protein (IAP) level;Using the ELISA method for determination of TH1 (TNF beta), TH2 cytokines (IL-4) level; Flow cytometry to detect CD4+ Th cells, Treg cells.Results 1. Healthy pregnant women not Trophinin adhesion molecule expression began in the early endometrial secretion, the strongest in the middle of the secretion of expression, secretion of late, and expressed in hyperplasia period did not see.2. Stop embryos development group Trophinin the expression of adhesion molecules, decreased significantly compared with normal pregnancy group, with significant difference (P< 0.01).3. The level of IAP embryonic development stops group was obviously higher than that of normal pregnancy group, with significant difference.4.Level of TNF beta in Embryonic development stops group increased compared with normal pregnancy group, with significant difference (P< 0.01); Embryonic development stops group of IL-4 levels higher than normal pregnancy group, with significant difference (p< 0.01).5. Stop embryos development group of TNF beta/IL-4 ratio higher than in normal pregnancy group, with significant difference (P< 0.01).6. Treg cell activity is abate, Th cells increased, the comparison between groups, with significant difference.ConclusionTrophinin adhesion molecule expression began in the early endometrial secretion and expression quantity is less, express the strongest mid, late secretion is reduced;Compared with normal pregnancy group and embryonic development stops group, found that after TingYu Trophinin in endometrial blood vessels and trophoblastic cells the expression of adhesion molecules than normal pregnancy group significantly decreased, thus affecting embryo in the womb lining on the initial adhesion, lead to stop embryonic development.We found that the embryo development stops the IAP levels in the blood of the patients with TNF-beta/IL-4 ratio were positively correlated, IAP can affect TH1, TH2 cytokines activity caused the imbalance between the cause embryo immune damage. Treg cells inhibition ability is reduced, excessive activation of Th cells, induce TH1 type (TNF beta), TH2 type cytokines (IL-4) storm, lead to stop embryonic development. Thus natural immune and adaptive immune cells dysfunction is involved in the onset of embryonic development stops process.