BTG1 Expression In Pancreatic Cancer And Its Clinical Significance

Objective:To investigate the expression of BTG1 protein in primary pancreatic ductal adenocarcinomas and to determine its prognostic significance. Methods:1. A total of 79 patients who had a pancreatic cancer resected between January 2005 and December 2014 at Fuzhou General Hospital were involved.Collecting the tissue specimens and their clinicopathologic informations.2. Immunohistochemistry is used to determine the protein expression of BTG1 gene in 79 surgically resected pancreatic cancer.3. All data were analyzed using SPSS22.0 software and P < 0.05 was considered statistically significant. The difference of BTG1 expression between tumor tissues and normal tissues and the relation between various variables and BTG1 expression in cancer tissue were analyzed by Chi-square test. Comparison of BTG1 expression status with overall survival in different subgroups of PC patients was performed using a log-rank correlation test and Cox regression test. Clinicopathologic variables with P< 0.05 on univariate analysis were selected in the multivariate Cox regression analysis. OS distributions were estimated using the Kaplan–Meier method. The overall survival was defined as the time from the date of confirmed diagnosis to death or the date of last telephone follow-up. Results:1. BTG1 positive expression was observed in 27.8%(22/79) of the tumor tissues, which was significantly lower than the 58.2%(46/79) of corresponding normal adjacent tissues by immunohistochemical staining(P<0.001).2. Through the stratified analysis, we found that the difference of BTG1 expression in four groups, PNI?T stage?lymph node metastasis and TNM stage,have statistical significance. With a positive expression in 16/35(45.7%) samples of PNI absent tumors and 6/44(13.6%) samples of PNI present tumors(P=0.002), a positive expression in 17/32(53.1%) samples of T1-2 tumors and 5/47(10.6%) samples of T3-4 tumors(P=0.000), a positive expression in 15/36(40.5%) samples of tumors without lymph node metastasis and 7/42(16.7%) samples of tumors with lymph node metastasis(P=0.018), a positive expression in 19/30(63.3%) samples of I-II stage tumors and 3/49(6.1%) samples of III stage tumors(P=0.000).3. There were two independent prognostic factors via multivariate analysis, including cell grade(hazard ratio 2.342, 95% confidence interval 1.417-3.873, P=0.001) and BTG1 expression status(hazard ratio 2.331, 95% confidence interval 1.104-4.924, P=0.027),respectively.4. Overall survival was better in PC cases with positive than negative BTG1 expression(P = 0.027). Conclusions:1. The decreased expression of BTG1 was detected in pancreatic cancer tissues.2. Patients whose pancreatic cancers had loss of BTG1 expression had worse overall survival after pancreatic cancer resection.