| Objective: By intraperitoneal injection of PCPA preparation insomnia animal model,extract serum melatonin and affect morpheus NPY as target,research the heavy moxibustion methods after the intervention effect on insomnia effect the change of the material basis,for the heavy moxibustion treatment of insomnia to provide powerful experimental basis.Methods: By intraperitoneal injection of p chlorophenylalanine(PCPA)method of the preparation of insomnia animal model in rats.Select the level 40 male health clean SD rats(200±10 g)completely random method is divided into five groups,7 days shows everything is normal,normal feeding with each rat experiment treatment:A control group,with normal rats as the negative control;B model control group,in experiment morning 8:00 received intraperitoneal injection PCPA liquid,1ML/100 g,once per day,for two consecutive days;C moxibustion group,began building in experiment morning 8:00,building method with the model control group,experimental 3d started to moxibustion treatment of heart,spleen,kidney acupuncture points,acupuncture points in cut hair,pour garlic into mud smear evenly on the prepared skin meridians,fixed with special cage,will refine moxa growth 8cm,diameter of4 mm moxa roll on acupoints of moxibustion,one-time moxibustion to burst fester,then single cage,no any during processing,until the moxibustion scar is off;D acupuncture group,began building in experiment morning 8:00,building method with the model control group,experimental 3d use press imported from Japan needle stick on the skin meridians heart,spleen,kidney,continuous 6d;E mild moxibustion group,began building in experiment morning 8:00,building method with the model control group,experimental 3d using special cage fixation,with refined mild moxibustion for skin points heart,spleen,kidney acupuncture points 15 min,consecutive 6d.The start process,respectively,for the first time treatment prior to the first building and materials before the three stages weigh the weight of rats,and record the data.Above after the experiment materials:anesthesia by intraperitoneal injection of rats with hydrate,operating scissors and tweezers open abdominal cavity,stripping out the abdominal aorta,3~5ml with disposable syringe pump arterial blood,3500 turn centrifugal,take the supernatant.Using the method of enzyme-linked immunosorbent(ELISA)to detect the content of serum melatonin and NPY.Using SPSS 20.0 statistical software for statistical processing of related experimental data,averaging,single factor variance analysis.Result: 1.PCPA abdominal cavity injection can make the normal rats sleep-wake up disorder,and for the injection of PCPA after 24 to 36 h,activity rhythm disorders,active during the day,some rats significantly restless mood,by observing with control group there were significant differences,remind replicate insomnia rats model of relatively method.2.Made before the mold for the first time,the weight of rats in a steady state,through the comparative analysis of no statistical difference(P>0.05);Before the treatment,for the first time after building groups of rats compared with the blank group rats weight analysis statistically significant(P<0.05);Before materials,model control group heavy moxibustion,acupuncture group,the mild moxibustion group by comparing statistically significant(P<0.05);heavy moxibustion,acupuncture group,the mild moxibustion group compared with blank control group with significant difference(P<0.05);heavy moxibustion and acupuncture group,the mild moxibustion group compared are significant differences(P<0.05);acupuncture group and mild moxibustion group is no statistical difference(P>0.05).3.Heavy moxibustion can significantly increase the PCPA result of insomnia rats serum melatonin and NPY levels,significant difference compared with model group(P<0.01).4.Acupuncture group can increase the PCPA result of insomnia rats serum melatonin and NPY levels,significant difference compared with model group(P<0.01).Compared with the heavy moxibustion group and the experimental results,there were significant differences(P<0.05)or less.5.Mild moxibustion group can increase the PCPA result of insomnia rats serum melatonin and NPY levels,compared with model group(P<0.01).Compared with the heavy moxibustion groups(P<0.05),and press the needle group was no significant difference(P>0.05).Conclusion: 1.The heavy moxibustion for insomnia model rats sleep function is obvious;2.PCPA abdominal cavity injection can make the normal rats sleep wake up disappear cycle,prompt PCPA building model preparation insomnia is more stable;3.Model after the success of the preparation of each group rats weight loss,heavy moxibustion weight adjustment is the most significant of insomnia rats3.The heavy moxibustion for insomnia rats sleep-wake regulation mechanism of circadian rhythm and its related to the regulation of melatonin;4.The experiments demonstrated strong insomnia associated with neuropeptide Y,it has to adjust the action of sleep,can also be measured in serum neuropeptide Y substances,and positively correlated with insomnia. |
PDF Full Text Request