| Objective： To establish on rats model of functionaldyspepsia,study the action mechanism of functional dyspepsia withChaishao gastritis particle therapy by observing on efficacy of Chaishaogastritis particles of functional dyspepsia rat model, and affecting onexpression of interstitial cells of Cajal in the rat antrum of Chaishaogastritis particles to functional dyspepsia model. Methods:60SD ratswere divided into6groups by the principle of randomization: blankcontrol group, model control group, high dose group Chaishao gastritisparticles, middle dose group Chaishao gastritis particles, low does groupChaishao gastritis particles,domperidone group (n=10). At the end ofsteel mesh plastic of cage,five per cage (n=2cage), feeding ratsseparately. The same cage of rats using the clip tail stimulation and madeirregular feeding mode14days,induced animal models of functionaldyspepsia modeling,rats were observed during the water intake and foodintake changes. After the modeling,with saline,Chaishao gastritis particles,domperidone interven the functional dyspepsia rat model to observechanges in water intake and food intake.14days later, to take theabdominal aorta blood to observe in rat plasma motilin levels, serumgastrin level, detection of gastric emptying rate,observed c-kit with immun ohistochemical assay, the ICC number of changes. To explore themechanism of functional dyspepsia with Chaishao gastritis particletherapies. Results:1.General observation: after the end of modeling, theblank control rats, water intake and food intake is more stable, nosignificant changes,the body weight compared with before modelingsignificantly increased; hair white, soft, luster, stool volume and odor nospecial change; good state of spirit. Model control rats, water intake andfood intake is gradually reduced, the weight gradually reduced trend;dirty yellow hair dry and dull, stool soft, more moisture, full of smellpungent; irascible, susceptible to irritation, and even biting each other,easily frightened, and each other gathered in the squirrel cage corner of;Chaishao gastritis particle group, domperidone group: before therapy, ratsgeneral situation with the model group rats no significant difference, aftertherapy, food intake, water intake, body weight significantly increasedcompared with the previous; hair no obvious change; Fecal dry，moisturecontent to reduce,full of the smell; temperament slightly docile,livelytussle significantly reduced.2. To observe rats with anatomy of have orwithout organic lesions of the stomach and duodenum: after the end ofmodel,the six experimental groups, each group were randomly selectedrats2, cut appetizer and duodenum,no observed stomach and duodenalmucosa, congestion and edema, erosion, bleeding, ulcer, uplift, and otherorganic disease, conform with endoscopic features of functional dyspepsia.3. Comparision of average daily water intake of rats in eachgroup:①Compared with the blank control group: average daily waterintake of model group, low dose group, middle dose group,high dosegroup and domperidone group was significantly lower than the blankcontrol group（P<0.01).②Compared with the model control group:average daily water intake of low dose group, middle dose group, highdose group and the group domperidone was significantly higher than themodel control group （P<0.01).③Compared with the low dosegroup:average daily water intake of middle dose group, high dose groupand the group domperidone was significantly higher than the low dosegroup（P<0.01).④Compared with the middle dose group:average dailywater intake of high dose group was significantly higher than themiddle dose group,the group domperidone was significantly lower thanthe middle dose group（P<0.01).⑤Compared with the high dosegroup:average daily water intake of the group domperidone wassignificantly lower than the high dose group（P<0.01).4. Comparisionof average daily food intake of rats in each group:①Compared with theblank control group: average daily food intake of model group, low dosegroup, middle dose group,high dose group and domperidone group wassignificantly lower than the blank control group（P<0.01).②Comparedwith the model control group: average daily food intake of low dosegroup, middle dose group, high dose group and the group domperidone was significantly higher than the model control group（P<0.01).③Compared with the low dose group:average daily food intake of middledose group, high dose group and the group domperidone wassignificantly higher than the low dose group（P<0.01).④Compared withthe middle dose group:average daily food intake of high dose group andthe group domperidone was significantly higher than the middle dosegroup（P<0.01).⑤Compared with the high dose group:average dailyfood intake of the group domperidone was significantly lower than thehigh dose group（P<0.01).5.Comparision of weight of rats in eachgroup:①Compared with the blank control group:weight of model group,low dose group, middle dose group,high dose group and domperidonegroup was significantly lower than the blank control group（P<0.01).②Compared with the model control group: weight of high dose group anddomperidone group was significantly higher than the model group（P<0.01).③Compared with the low dose group:weight of high dosegroup and domperidone group was significantly higher than the low dosegroup（P<0.01).④Compared with the middle dose group:weight of highdose group and the group domperidone was significantly higher than themiddle dose group （P<0.01).⑤Compared with the high dosegroup:weight of the group domperidone was significantly lower than thehigh dose group（P<0.01).6.Comparision of gastric emptying rate ofrats in each group:①Compared with the blank control group: gastric emptying rate of model group,low dose group, middle dose group anddomperidone group was significantly lower than the blank control group（P<0.01).the high dose group and the blank control group of gastricemptying rate was no significant difference（P>0.05）.②Compared withthe model control group: gastric emptying rate of low dose group, middledose group, high dose group and the group domperidone wassignificantly higher than the model control group（P<0.01).③Comparedwith the low dose group:gastric emptying rate of middle dose group, highdose group and the group domperidone was significantly higher than thelow dose group （P<0.01).④Compared with the middle dosegroup:gastric emptying rate of high dose group and the groupdomperidone was significantly higher than the middle dose group（P<0.01).⑤Compared with the high dose group:gastric emptying rateof the group domperidone was significantly lower than the high dosegroup（P<0.01).7.Comparision of Motilin of rats in each group:①Compared with the blank control group: Motilin of model group wassignificantly lower than the blank control group（P<0.01),Motilin ofhigh dose group significantly higher than the blank control group（P<0.01).②Compared with the model control group: Motilin of lowdose group, middle dose group, high dose group and the groupdomperidone was significantly higher than the model control group（P<0.01).③Compared with the low dose group:Motilin of high dose group was significantly higher than the low dose group（P<0.01),themiddle dose group, the domperidone group and the low dose groupMotilin was no significant difference（P>0.05）.④Compared with themiddle dose group:the high dose group, the domperidone group and themiddle dose group Motilin was no significant difference（P>0.05）.⑤Compared with the high dose group:Motilin of the group domperidonewas significantly lower than the high dose group （P<0.01).8.Comparision of Gastrin of rats in each group:①Compared with the blankcontrol group: Gastrin of model group, low dose group, middle dosegroup and domperidone group was significantly lower than the blankcontrol group（P<0.01),the high dose group and the blank control groupMotilin was no significant difference（P>0.05）.②Compared with themodel control group: Gastrin of low dose group, middle dose group, highdose group and the group domperidone was significantly higher than themodel control group （P<0.01).③Compared with the low dosegroup:Gastrin of high dose group was higher than the low dose group（P<0.05),the middle dose group, the domperidone group and the lowdose group Gastrin was no significant difference（P>0.05）.④Comparedwith the middle dose group:Gastrin of high dose group was higher thanthe middle dose group（P<0.05),the domperidone group and the middledose group Gastrin was no significant difference（P>0.05）.⑤Comparedwith the high dose group:Gastrin of the group domperidone was significantly lower than the high dose group（P<0.01).9.Comparision ofthe gray value of the ICC-positive cells in antral organization of rats ineach group:①Compared with the blank control group: the gray value ofthe ICC-positive cells in antral organization of model group, low dosegroup, middle dose group,high dose group and domperidone group wassignificantly lower than the blank control group（P<0.01).②Comparedwith the model control group: the gray value of the ICC-positive cells inantral organization of low dose group, middle dose group, high dosegroup and the group domperidone was significantly higher than the modelcontrol group（P<0.01).③Compared with the low dose group:the grayvalue of the ICC-positive cells in antral organization of high dose groupwas higher than the low dose group（P<0.05),the middle dose group, thedomperidone group and the low dose group of the gray value of theICC-positive cells in antral organization was no significant difference（P>0.05）.④Compared with the middle dose group:the gray value ofthe ICC-positive cells in antral organization of high dose group washigher than the middle dose group（P<0.05).the domperidone group andthe middle dose group of the gray value of the ICC-positive cells in antralorganization was no significant difference（P>0.05）.⑤Compared withthe high dose group:the gray value of the ICC-positive cells in antralorganization of the group domperidone was significantly lower than thehigh dose group（P<0.01).Conclusion:1." Tail clamp stimulation "and " irregular feeding " joint model is just only " tail clamp stimulation "or"irregular feeding " of FD rat model of improvement, able to replicate theideal FD rat model.2. The gray value of the ICC-positive cells in antralorganization of model group, low dose group, middle dose group anddomperidone group was lower than the high dose group，it’s means thehigh dose group, it’s means high dose group to increase the number ofthe gray value of the ICC-positive cells in antral organization comparedwith the mode control group, the low dose group, middle dose group anddomperidone group is better.3.High dose group of the Chaishao gastritisparticles of functional dyspepsia rat model’s motilin, gastrin and gastricemptying rate compared to the model control group, low dose group,middle dose group, domperidone group is better, it shows that the highdose group can be better to promote gastrointestinal motility.4.Thepossible mechanism of the treatment of Chaishao gastritis particles ofFD rats is，it influences the FD rat gastric antrum’s ICC, increasesthe number of antral the number of ICC, so improve in motilin andgastrin secretion, enhanced gastrointestinal motility, thereby promotinggastric emptying. |
PDF Full Text Request