The Synthesis Of MiRNA-122 Small Molecule Modulators And Study Of Anti-hepatoma Cell Function

Objective:Hepatocellular carcinoma (HCC) is one of leading causes of death in the world. Short survival time and poor prognosis of patients with hepatocellular carcinoma. Therefore, early prevention and treatment of liver cancer is particularly important. Hepatitis C virus is a major cause of cirrhosis and liver cancer. Early intervention is very effective and important. miRNA-122 is a specific microRNA in liver cancer, which can be highly expressed and stable in normal liver cells. High expression of miRNA-122 can inhibite liver cancer cell’s invasion and metastasis, but also protect the genetic material of the hepatitis C virus.Purpose:This study aims to design and synthesize some new compounds. We hope that these compounds are capable of liver cancer and hepatitis viruses play a therapeutic role.Experimental Design and Methods:In this experiment, a total of 12 compounds were synthesized, including 11 new derivatives. Measured two tumor cell lines (HuH-7 cell lines and HepG2 cell lines) to the drug sensitivity and inhibition rate by MTT assay.Results & Conclusions:There are two compounds (Compound 7, Compound 8) in MiRNA-122 high expression HuH-7 hepatoma cell lines exhibited growth inhibition of tumor cell. One compound (Compound 9) has played a role in promoting tumor cell growth, and they are significantly different (P<0.05 or P<0.01). In contrast, MiRNA-122 low expression in HepG2 cells was not observed a significant difference between the phenomenon of inhibition or promotion. Accordingly, we can depending on the experimental results and the functions and distribution of MiRNA-122, By establishing the development of individualized treatment programs, the application of the above compound to treat tumor cells and hepatitis C virus. Or developed more efficient compound drugs.