Anti-HBV Study Of Styela Plicata (Lesucur)

Styela plicata is an ascidian which belongs to class Ascidiacea, subphylum urochordata, phylum Chordates. About 2000 kinds of ascidians have been found in the nature and they distribute over the maritime space. Studying on bioactive components from ascidian has been one of the most popular domains in marine drug research since the eighties of the twentieth century. Many chemical compositions such as peptide, alkaloids, macrolide, terpenoid, and so on, have been found in ascidian. Most of them have strong biological activity, such as anti-tumor, anti-virus, antibiosis, immunity modulation and biocatalysis, etc.There are abundant ascidian resources in the South China Sea. Styela plicata is one of these. In recent years, we have carried out the basic research on the marine organism. The alcohol extract of the animal has a definite inhibitory effect on the secretion of hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) in 2.2.15 cells, although the exact mechanism is not known. For evaluating the anti-HBV activity and finding out the anti-HBV mechanism of Styela plicata farther, we made a systemic pharmacodynamics study on the alcohol extract of Styela plicata. The whole task has 3 parts.1 Serology study of alcohol extract from Styela plicata on inhibiting HBV in vitroThis task was carried out according to the guideline diagnostic serology. Weanalyzed the results of the alcohol extract from Styela plicata to HBsAg, HBeAg,anti-HBs and anti-HBe, and evaluated the anti-HBV effect of it. The antiviral functionof the alcohol extract from Styela plicata was speculated.