| Backgroud:Coronary heart disease (CHD) is a chronic inflammatory disease, this view has been recognized, and a variety of inflammatory factors involved in the process of CHD. TIPE2 is a kind of negative immune regulatory factor, has an important value to maintain Immunologic homeostasis. Therefore TIPE2 involved in various inflammatory disease progress, Studys shows that TIPE2 also play an important role in the process of atherosclerosis, but the specific mechanism is unclear.Objective:TIPE2 is a new found negative immune regulatory factor, and play an anti-atherosclerosis role in the process of coronary heart disease (CHD). This study was to explore the role of TIPE2 in the pathogenesis of coronary heart disease (CHD) and the association between related inflammatory cytokines and TIPE2 levels in patients with coronary heart disease (CHD), to further analyze the ignaling pathways by which TIPE2 play the anti-atherosclerosis role.Methods:Choosen 100 cases of patients with coronary artery stenosis determined by coronary angiography examination between December 2010 and November 2010 in Affiliated Hospital, Binzhou Medical College, who were divide into single lesion, double-vessel and multiple vessel groups, and 30 cases of non-CHD at the same time line. Branch of coronary artery lesions using the following criteria:left main lesion, descending branch and (or) before its branch lesions, left circumflex branch and/or its branch lesions, right coronary artery and (or) its branch lesions, the above any other lesions, all count as a pathological changes. Using polymerase chain reaction (PCR) assay the TIPE2 mRNA level in peripheral blood mononuclear cells, with ELISA method for determination the expression of VCAM-1, TNF-α,MMP-9, MCP-1, and interleukin-1,6,8 levels in serum.Results:1) TIPE2 mRNA levels in the peripheral blood mononuclear cells measurement results:TIPE2 mRNA level in patients with coronary artery lesions was lower than that in non-CHD group.In CAD groups, the levels of TIPE2 are increasing with the increase of the coronary artery lesion counts:single-vessel lesions group vs double-branch lesions group(P< 0.01), single-vessel lesions group vs multi-vessel lesions group (P<0.01), double-branch lesions group vs multi-vessel lesions group (P < 0.01); 2) The levels of VCAM-1, MMP-9, MCP-1, TNF-α, interleukin-1, 6,8cytokines have significant difference between the non-CHD and CHD groups; 3) Using multiple linear stepwise regression analysis the assosiation between the level of TIPE2 mRNA in PBMCs and the level of inflammatory factors in patients with coronary heart disease (CHD).such as VCAM 1, MMP-9, MCP-1, TNF alpha cytokines, interleukin 1,6,8. Five model is established, the fifth model R= 0.907, standard error is 0.667, R value is maximum, VCAM 1, MMP-9, MCP-1, interleukin 1,6 cell factor are into the model 5, F= 242.633, P< 0.001, suggesting the regression equation is established, the equation is Y= 27.316-0.018X1-0.084X2-0.054X3-0.012X4-0.007X5 (XI for MMP-9, X2 for IL-6, X3 for IL-1, X4 for MCP-1, X5 for VCAM-1. Seeing from standardization coefficient of symbols, VCAM-1, MMP-9, MCP-1, interleukin 1 and 6 were negatively correlated with the TIPE2 mRNA levels.Conclusion:This sduty prompt that TIPE2 is involved in the pathogenesis of coronary heart disease (CHD), and play the role of anti-atherosclerosis. And the mechanism may be by adjusting the related factor VCAM 1, MMP-9, MCP-1 the levels of cytokines, interleukin 1,6. Further research could focus on whether could be prevent the initiation and progression of atherosclerosis by excited TIPE2 signaling pathway. |
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