| Background and Objective Apolipoprotein E(Apo E) is an important functional protein closely related with lipid metabolism and an important component of lipoproteins. Moreover, Apo E possesses gene polymorphism with a variety of physiological functions, which is regarded asthe dangerous genetic factor leading to atherosclerotic stenosis. However, intracranial atherosclerotic stenosis is exactly the main cause of senile cerebral infarction patients. In this paper, it aims to investigate the effects of Apo E gene polymorphism on the pathological changes of cerebral artery stenosis in elderly patients with blood lipid metabolism and atherosclerotic cerebral infarction.Methods 1, At the Second Affiliated Hospital of Zhengzhou University, the department of geriatrics patients are randomly selected 180 persons in December 2013 to September 2015, male 110 cases, female 70 cases, ages vary(82.28 ± 6.28), who are diagnosed as atherosclerotic cerebral infarctionvia cranial magnetic resonance angiography and(or) transcranial doppler ultrasonography; 2, Expand the specific gene fragment by PCR amplification instrument and use the method of“Gene Chip”, then read the Apo E genotype phenotype determining gene fragment to go on with group comparison; 3, Apply the automatic biochemical analyzer to test sample of plasma low density lipoprotein cholesterol(Low density lipoprotein cholesterol, LDL-C), total cholesterol(Total, Cholesterol, TC),the concentration of glycerin three greases(Triglyceride, TG) and high density lipoprotein cholesterol(High density lipoprotein cholesterol, HDL-C) for statistical analysis, and analyze the relationship with Apo E gene polymorphism; 4, Use 1.5T head magnetic resonance angiography(MRA) to examine and determine the intracranial artery(anterior cerebral artery, middle cerebral artery, posterior artery, vertebral basilar artery), then determine whether is atherosclerosis and stenosis degree or not.According to the NASCET classification: normal group, there is no vascular atherosclerotic stenosis; atherosclerosis group,there is atherosclerosis formation and no vascular stenosis; mild stenosis, the percentage of atherosclerosis and stenosis is under 50 percent; moderate stenosis group, the percentage of vascular stenosis ranges from 50 percent to 69 percent; severe stenosis group, the percentage of vascular stenosis ranges from 70 percent to 99 percent; occlusion group, there is no blood vessels or no blood flow signal.This phenomenon can eliminate congenital vascular exclusion abnormal and perform statistical analysis with gene polymorphism and blood lipid concentration. Statistical methods: measurement data can manifested with mean ± standard( ±S). The comparison of group mean samples can adopt single factor variance. Qualitative data can be tested by chi square and inspection standard is a = 0.05, P<0.05 difference is statistically significant. Statistical analysis was performed using SPSS20.0 software package. XResults the Apo E allele type(frequency): epsilon 2(25.6%), epsilon 3(65.6%), epsilon4(8.9%), genotype(frequency): homozygous ?2/?2(1.1%), ?3/?3(62.2%), ?4/?4(5.6%), miscellaneous zygote ?2/?3(24.4%),?2/?4(3.3%),?4(1.1%). Epsilon 2 group(?2/?2 and ?2/?3), epsilon 3 group(?3/?3 and ?2/?4), epsilon 4 group( ?3/?4 and ?4/?4) TC, TG, LDL-C levels increase in turn, epsilon4 group TC, LDH-C and epsilon2 group, epsilon3 groups have statistical significance(P<0.05) and TG has no significant difference; HDL-C concentration is decreased one by one withepsilon4 group statistical analysis(P<0.05), According to blood lipid and atherosclerosis stenosis rate statistical analysis, TC, TG, LDL-C concentration increase and statistical analysis P<0.05 with the pathological aggravating; genotype and vascular analysis.Compared with group epsilon 3, the rate of epsilon 4 groups of patients with artery stenosis is higher and epsilon 2 group arteriosclerosis stenosis is low, the results have statistical significance(P< 0.05).Conclusion 1.Apo E gene possesses polymorphism.Allele and genotype are respectivelyepsilon 2(?2/?2, ?2/?3), epsilon 3(?3/?3, ?2/?4), epsilon 4(?3/?4, ?4/?4), which epsilon 3 and ?3/?3 frequency are the highest as 65.6% and 62.2%, epsilon 2 and epsilon 4 are at the bottom. 2.Apo E gene polymorphisms are involved in lipid metabolism. Besides, the effects of epsilon2 and epsilon 4 are obvious. Between them, epsilon 2 can reduce TC and LDL-C, HDL-C, but epsilon 4 is on the contrary. 3.Blood lipid metabolism has a certain correlation with atheroscleros and the atherosclerotic stenosispathological aggravating, TC and LDL-C levels increase and HDL-C decreases(P<0.05). 4.Apo E and intracranial artery atherosclerosis stenosis are closely related. Allelic gene epsilon4 is the dangerous factor of intracranial arterial stenosis. However, epsilon 2 has a protective effect. |
PDF Full Text Request