Abnormality And Significance Of Th9 Cells And IL-9 In Peripheral Blood Of Patients With Primary Nephritic Syndrome

Objective To detect the expression of T helper cells 9(Th9) in the peripheral blood and the level of interleukin(1 L)-9 in the plasma of primary nephrotic syndrome(PNS) patients, and to analyze the relationship between Th9 and the pathogenesis of PNS, and their possible role in the immunological pathogenesis of PNS were explored.Methods Thirty-five patients with PNS and 30 healthy controls were included. Th9 cells were obtained from PNS patients and healthy controls and detected by flow cytometry. The levels of IL-9 in PNS patients and healthy controls were detected by ELISA tests. T-test and Spearman's correlation were used for statistical analysis.Results The expression of Th9 of PNS patients were significantly higher than normal controls [(1.06�0.20)%, (0.26�0.07)%, respectively, P<0.01]. Meanwhile, The level of IL-9 of PNS patients were significantly higher than normal controls [(70.02�13.66)pg/ml, (18.77�5.35)pg/ml, respectively, P<0.01]. There were positive correlations between Th9 and 24-hour urinary protein, cholesterol, triglyceride, blood urea nitrogen, the level of IL-9 respectively, and Th9 cells were inversely proportional to serum albumin, Immunoglobulin G respectively. There were positive correlations between IL-9 and 24-hour urinary protein, cholesterol, triglyceride respectively, blood urea nitrogen, and the level of IL-9 were inversely proportional to serum albumin, Immunoglobulin G, C3 respectively.Conclusion High expression of Th9 and level of IL-9 maybe involved in the pathogenesis of PNS. And is related with clinical indicators. These results indicate that the abnormality of Th9 my play an important role in the pathogenesis of PNS.