1、Compare The Polymorphism Of TIM-1 Gene Locus And The Level Of IL-13 With PNS In Children Between The Han Nationality And Moggol Nationality 2、Association Between TIM-3 Gene Polymorphisms And IFN-r With Primary Nephrotic Syndrome In Children Between

FirstObjective To the genetic association between the TIM-1 polymorphisms and primary nephrotic syndrome(PNS)in children,to assess the effect of IL-13 on immunologically mediated renal injury in PNS and its diagnostic significances, assess Methods A case-control study was performed in 20 cases of PNS patients (during 2014.10-2015.12)and 40 cases of healthy controls.including the Han nationality and Moggol nationality.Inclusion criteria:cases PNS diagnosis standard,according to our country diagnoses in children with PNS. Control group except allergic disease,kidney disease and family history of kidney disease,the recent history of immunosuppressant application.Sbjecting peripheral venous blood samples 2ml in each tube.Associations between the TIM-1 polymorphisms and PNS were examined by using polymerase chain reaction-restriction fragment length polymorphism analysis,PCR-polyacrylamide gel electrophoresis and gene sequencing technology, capillary electrophoresis analysis,genotype and allele frequencies were calculated.the relationship was found between comparison groups.Using enzyme-linked immunosorbent to assay the level of IL-13.Analysis of data:application of data from the correspondind statistical methods and software.Each genetype and frequency results are obtained.Inspection level for a=0.05,when P<0.05,the difference was statistically significant,the opposite is meaningless.Results The genotype frequencies of G/G,A/G,A/A in the TIM-1 gene-1454G/A region were 0.905,0.095, O.OOin PNS chidren and0.55,0.4,0.05 inthe Han nationalityhealthy children and 0.6,0.35.0.05 in the Moggol nationality healthy children.There was significiant difference in genotype frequnencies between patients and the Han nationality healthy control controls((x2=5.64, P<0.025)There was no significiant difference in genotype frequnencies between the different healthy control controls((x2=0.11, P>0.05).Carriers of the-1454G/A single-nucleotide polymorphism(SNP) were increased among PNS patients as compared with controls (OR=6.23,95%CI= 1.38-28.22, P=0.01)) The IL-13 content of PNS patients with acute stage than the control group was statistically significantly different(X2=48.57, P=0.000 <0.05).There was no difference between the normal controls of the Monglian and Han nationality in comparison with the level of IL-13.Conclusion:TIM-1-1454G/A gene polymorphism may be associated with the onset of Inner Mongolia region of the Han nationality children about the disease of PNS.There was no difference between the normal controls of the Monglian and Han nationality in comparison of TIM-1 1454G/A;The level of IL-13 IS related to the onset of PNS.TIM-1 gene polymorphism and IL-13 levels involved in onset of PNS in children.Specific regulation pathway between still needs further research.SecondObjective:To assess the genetic association between the TIM-3 polymorphisms and primary nephrotic syndrome(PNS)in children,to assess the effect of interferon-r(IFN-r) on immunologically mediated renal injury in PNS and its diagnostic significances.Methods A case-control study was performed in 20 cases of PNS patients and 40 cases of healthy controls.Associations between the TIM-3 polymorphisms and PNS were examined by using polymerase chain reaction-restriction fragment length polymorphism analysis,PCR-polyacrylamide gel electrophoresis and gene sequencing technology, capillary electrophoresis analysis,genotype and allele frequencies were calculated.the relationship was found between comparison groups.Results The genotype frequencies of A/A,A/C,C/C in the TIM-3gene -574A/C region were 30%,45%,25%in healthy chidren and9.5%, 28.6%,61.9% in children with PNS.There was significiant difference in genotype frequnencies between patients and controls(x,2=7.08,P=0.05).Carriers of the -574A/C single-nucleotide polymorphism(SNP) were increased among PNS patients as compared with controls (OR=4.875, ORL:1.58, ORu=14.1; P=0.05) There was sinificant difference in genotype distribution between patients and controls.(x2=7.08,P=0.05); The frequency of the allele genes -574C/C increased than others. (OR=4.875, ORL=1.58, ORu=14.1; P=0.05); To test the level of IFN-γbetween the children who are healthy and the PNS patients.To analyze its relationship with PNS clinical indicators.There is no difference between PNS group and normal group during the level of IFN-y.The pathgenesis of PNS in children is probablly related to the TIM-3-574A/C gene polymorphisms.