Expression And Significance Of FTO In Liver Biopsy Tissue With Nonalcoholic Fatty Liver Disease

BackgroundIn recent years,with the rapid development of the national economy,people's diet and lifestyle also has the very big change,obesity has increased rapidly,and in obese people,the rates of nonalcoholic fatty liver disease(NAFLD) can be as high as 75%,preliminary estimates on a global scale the proportion of NAFLD may reach 15%-30%. Fat mass and obesity associated gene(FTO) is the first to be clear with obesity in recent years as a new type of candidate genes,which have extremely close relation with obesity.A large number of research results show that the FTO was associated with the metabolic syndrome,type 2 diabetes,coronary heart disease and other metabolic diseases,is the one of the independent risk factors for disease and related deaths.But the relationship between FTO and NAFLD and its mechanism of action research is relatively small,in patients' liver with NAFLD how FTO distribution,express how much and whether FTO associated with NAFLD,it is not very clear.This study designed to detect the expression of FTO protein in the liver biopsy tissue with nonalcoholic fatty liver disease by using immunohistochemical,explore the role of FTO in the occurrence and development of NAFLD. ObjectiveTo study the expression of the fat mass and obesity associated gene in patients' liver biopsy tissues with nonalcoholic fatty liver disease,exploring the FTO role in the occurrence and development of NAFLD. MethodsSelected the patients treatment and under ultrasound guided percutaneous liver puncture from January 2010 to December 2014 in the first affiliated hospital of zhengzhou university hospital, retrospectively analyzed the clinical and pathological data. Immunohistochemical SP ldpe-g-nvp detect the expression of FTO protein in liver biopsy tissue, and compare the clinical and biochemical index such as blood lipid, blood glucose, liver enzyme. At the same time, calculated and compared the parameters of obesity(BMI and WHR), insulin resistance parameters(HOMA-IR) and insulin sensitivity index( ISI), etc. Measurement data to mean + /- standard deviation(x±s) said, multiple sets of comparison use the Kruskal- Wllis inspection, further comparison between the two groups use the Mann- Whitney U test( or Bonferroni method), correlation between the two data uses Spearman correlation analysis, P < 0.05 for the difference was statistically significant. ResultsSelected the total 151 cases of patients, including 20 cases with other liver diseases( no steatosis or adipose sex hepatitis) as normal control group( 15 cases of male, 5 cases of female); 131 cases of NAFLD diagnosed by imaging and histopathological criteria [ including nonalcoholic simple fatty liver group( NAFL) 56 cases( 34 cases of male, 22 cases of female) and nonalcoholic steatohepatitis group( NASH), 75 cases( 46 cases of male, 29 cases of females) ].(1)Compared normal control group with NAFL, NASH group, the differences in gender, age composition between the three groups have no statistical significance( P>0.05);(2)Compared with the control group, patients with NAFL, NASH group of BMI, WHR, FINS, TG, ALT, AST, GGT, hs CRP level and HOMA-IR significantly increased( P<0.001), HDL, ISI significantly reduced( P<0.001); Compared with the control group, the NASH group of the HbA1 c, ALP, LDL,TC were higher( P<0.05); Compared with NAFL group, NASH set of lower level of HDL-cholesterol( P<0.05), ISI significantly decreased(P<0.01), LDL, ALT, AST, GGT were elevated( P<0.05), FPG, FIns, HOMA-IR, TG, TC significantly increased( P<0.01);(3)FTO protein expression in the liver generally, histological localization primarily in the liver cell cytoplasm, rare in the nucleus. Compared with normal control group, the integral optical density( IOD) of FTO protein positive expression were significant increased in NAFL group and NASH group, and the difference with statistical significance( P<0.001); and as the deterioration of the degree of liver steatosis and inflammation, FTO protein expression has a tendency to rise, but there was no statistical significance( P>0.05).(4)FTO protein expression and the BMI and WHR were significantly positive correlation( P<0.001), and FIns, HOMA IR, TG, hs-CRP were positively correlated( P<0.05); and negatively correlated with ISI, HDL-C( P<0.05). ConclusionsThe expression of FTO protein in liver biopsy tissue with nonalcoholic fatty liver disease was significantly higher than that of the control group, and there has a certain relevance between FTO expression quantity and sugar, lipid metabolism and various indexes such as obesity parameters with NAFLD, suggesting FTO might participate in the occurrence and development of NAFLD by interfering with the body's sugar, lipid metabolism and increasing the insulin resistance.